苹果内吸磷残留量的动态酶化学研究

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用Hestrin胆硷酯酶活性测定法,系統研究以不同方式施用内吸磷(E-1059)后,苹果内吸磷残留量的动态变化情况。施药方式有三种:(1) 浸果—仅对树上的苹果进行药浸,树叶和树枝不接触药液。(2) 噴叶—苹果以塑料膜包好后向树上嘖药,只树叶和枝上着药,而苹果不着药。(3) 浸枝——将带有果和叶的一段树枝剪下,断端浸入药液中迫使吸收药液24小时。检测結果:浸果施药后第一天内,苹果可检测出很高的内吸磷残留量(可达2 ppm左右),1天后残留量降到0.75 ppm以下,两周后残留量可降低到接近零;噴叶施药后12天内,經数次检测,均未发现有内吸磷残留;用药液浸枝,虽然第一天树枝吸收了不少的药液,但苹果中几乎检測不出内吸磷的残留,第二天换以水浸枝,却反而检测出内吸磷残留量(将及0.5 ppm),大約10天左右残留量降到接近零。試驗結果说明:一般噴雾时内吸磷的残留主要是由于药液直接接触苹果果皮所致,通过叶和枝内吸輸导至苹果上的药液是微乎其微的;浸果施药后第一天内残留量最高,以后逐漸降低,約两周后降到接近于零;浸枝时迫使吸入药液,但輸导至苹果上的药物甚少,并且内吸輸导需要一定时間,所以第二天残留量最高。关于苹果施用内吸磷的期限,似乎可职适当后延至收获前3或4周。 The Hestrin cholinesterase activity assay was used to systematically study the dynamic changes of phosphorus uptake in apple after phosphorus uptake (E-1059) in different ways. There are three ways to apply: (1) Dip fruit - Dip the apple on the tree only, leaves and branches do not touch the liquid. (2) Spraying leaves - Apple wrapped in plastic film to the tree after the medicine, only the leaves and branches of medicine, and Apple can not medicine. (3) Soaking branches - a section of the branches with fruit and leaves cut off, immersed in the liquid end of the solution to force liquid absorption for 24 hours. Test results: Apple can detect very high levels of phosphorus uptake (up to 2 ppm) on the first day after immersing in fruit. After 1 day, the residual amount drops to below 0.75 ppm, and the residue can be reduced after two weeks To nearly zero; Spraying leaves within 12 days after testing, after several tests, were found to have Phosphorus residue; with liquid soaked branches, although the first day branches absorbed a lot of liquid, but almost all the detection of apples No residue of phosphorus in the next day instead of flooding, but instead detected phosphorus residues (and 0.5 ppm), about 10 days or so the amount of residue dropped to nearly zero. The results showed that the residual phosphorus uptake during spraying was mainly caused by the direct contact of the liquid with the apple peel, and the liquid introduced to the apple through the leaves and branches was negligible; the first day after immersing The amount of residual within the highest, and then gradually decreased after about two weeks after the drop to close to zero; soaking branches forced inhalation of liquid, but the little medicine is delivered to the apple, and the internal absorption conduction takes some time, so the Two days maximum residue. As for the duration of phosphorus uptake in apples, it seems that the appropriate postponement may be postponed to 3 or 4 weeks prior to harvest.
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