论文部分内容阅读
目的:用电子顺磁共振(ELECTRONPARAMAGNETICRESONANCE,EPR)直接测定自由基的方法观察在高压氧(HBO)下暴露后大白鼠肺组织内自由基的消长规律.方法:动物在300KPA氧压下分别暴露105、161、214和262分钟.动物出舱后,腹腔注射自旋捕捉剂??α-苯基-N-叔丁基甲亚胺-N-氧化物(N-T-BUTYL-α-PHENYLNITRONE,PBN),用EPR波谱仪测定肺内维生素C自由基(V?C),用化学比色方法检测肺内维生素C(VC)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性.结果:HBO暴露后,肺组织内V?C浓度升高,且V?C生成随暴露时间的延长而增加.161分钟时,这种增加已非常显著(P<0.001),在105分钟时,VC量下降,SOD活性升高却非常明显(P<0.001);至262分钟时,V?C增加幅度略低于214分钟暴露,而事先注射VC可明显提高肺内V?C浓度(P<0.001),但肺组织内VC量未见增加.MDA只在用VC处理的大白鼠在262分钟时有明显升高(P<0.05).结论:HBO暴露时,肺组织内自由基生成量随所用氧压-时程值的增加而增多,同时,肺组织内VC“,”Aim: The changes of free radicals generation in the lung tissue of rats exposed to 300kPa oxygen were observed using the electron paramagnetic resonance(EPR) direct free radical measuring method. Methods: The rats were exposed to 300kPa oxygen for 105、161、214 and 262min respectively. EPR spectroscopy was applied and spin trap?N?t?butyl?α?phenylnitrone(PBN) was used to measure the free radicals in the lung tissue. Meantime, the ascorbic acid(V?C), MDA and SOD activity were measured with spectrometry. Results...