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目的 确立血脾屏障的结构 ,探讨其在脾脏免疫反应中的作用。方法 90只健康昆明种小白鼠 ,随机分成三组 ,A组于尾静脉注射炭粒混悬液 ,B组注射新型隐球菌混悬液 ,C组注射炭粒+新型隐球菌混悬液 ,注射后分别于 3h ,1d ,3d ,7d ,14d ,2 8d ,取脾组织作光镜和电镜观察。结果 3h组炭粒和细菌绝大多数以游离状态存在于边缘区的脾窦内 ,白髓未见炭粒和细菌 ,1～ 3d被边缘区脾窦内皮细胞和巨噬细胞大量吞噬 ,3d以后吞噬炭粒的巨噬细胞向滤泡中心迁移 ,2 8d白髓内仍见少量散在的吞噬炭粒的巨噬细胞。结论 血脾屏障位于边缘区 ,主要由内皮细胞及其基底膜、巨噬细胞、网状纤维等构成 ,其功能是通过机械屏障和生物屏障作用维持白髓内在微环境的恒定 ,并通过巨噬细胞向白髓传递抗原信息。
Objective To establish the structure of blood-spleen barrier and explore its role in spleen immune response. Methods Ninety healthy Kunming mice were randomly divided into three groups: group A was injected with the suspension of charcoal particles in the caudal vein; group B was injected with C. neoformans suspension; group C was injected with charcoal + After 3h, 1d, 3d, 7d, 14d, 28d respectively, the spleen tissues were taken for light and electron microscope observation. Results Most of the carbon granules and bacteria in the 3h group were present in the splenic sinus of the marginal zone in free state. No carbon granules and bacteria were found in the white pulp, and 1 ~ 3d was swallowed extensively by the splenic sinusoidal endothelial cells and macrophages in the marginal zone. Macrophages engulfing the carbon particles migrate to the follicular center, a small amount of scattered macrophages engulfing the carbon particles are still seen in the white marrow within 28 days. Conclusion The blood-spleen barrier is located in the marginal zone and is mainly composed of endothelial cells and its basement membrane, macrophages and reticular fibers. Its function is to maintain the intrinsic microenvironment of the white pulp through mechanical barrier and biological barrier, Cells transmit antigen information to white blood cells.