花生果针入土后16天(16 DAP),种子干重和鲜重开始迅速增加。整个发育阶段可分为5个时期:组织分化期(0～20 DAP)、成熟前期(21～28 DAP)、成熟中期(29～40DAP)、成熟中后期(41～62 DAP)和成熟后期(63～88DAP)。种子发芽率在成熟前期和中期迅速提高并到达最大值,而苗成活率在成熟中后期达到最大值,苗鲜重则以88 DAP种子的为最大。种子发育过程中,贮藏蛋白质的合成与积累模式与种子干重变化相似。SDS-PAGE分析表明,种子发育初期(16 DAP)子叶中已积累花生球蛋白和伴花生球蛋白I。双向凝胶电泳显示花生球蛋白各个亚基在20DAP时均已存在,伴花生球蛋白I的主要亚基在整个发育过程中其等电点有所变化,含量也逐渐增加。其他蛋白质在种子发芽力形成阶段(20～40 DAP)的变化较为显著。 After 16 days of peanut needles penetration (16 DAP), seed dry weight and fresh weight began to increase rapidly. The whole developmental stage can be divided into five stages: 0 ~ 20 DAP, 21 ~ 28 DAP, 29 ~ 40DAP, 41 ~ 62 DAP, 63 ~ 88DAP). The seed germination rate rapidly increased at the early and middle stages of maturity and reached the maximum, while the seedling survival rate reached its maximum at the middle and late stages of maturity, while seedling fresh weight was the largest at 88 DAP seeds. During seed development, the pattern of storage and accumulation of stored protein was similar to that of seed dry weight. SDS-PAGE analysis showed that arachidin and spheroplatin I had accumulated in the cotyledons of early seed development (16 DAP). Two-dimensional gel electrophoresis showed that each subunit of peanut globulin existed at 20DAP. The major subunit of peanut globulin I changed its isoelectric point during the whole development, and its content also gradually increased. The changes of other proteins in seed germination stage (20 ~ 40 DAP) were significant.