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为阐明表皮生长因子（ＥＧＦ）对卵巢功能的调节作用，本实验用培养的大鼠卵巢颗粒细胞观察了ＥＧＦ与人绒毛膜促性腺激素（ｈＣＧ）共同作用对细胞增殖、甾体激素与ｃＡＭＰ生成的影响。结果：ＥＧＦ可明显抑制ｈＣＧ刺激的孕酮生成，并呈剂量依赖性，而对ｈＣＧ刺激的雌二醇生成无影响；ＥＧＦ对ｈＣＧ刺激的颗粒细胞ｃＡＭＰ生成具有增强作用，使ｃＡＭＰ生成增加；ＥＧＦ能刺激颗粒细胞增殖，使３ＨＴｄＲ掺入增加，ｈＣＧ不影响ＥＧＦ的这种作用。提示ＥＧＦ与ｈＣＧ对卵巢功能的影响既有相互作用，又有各自的作用。
In order to clarify the regulatory effect of epidermal growth factor (EGF) on ovarian function, we observed the effect of EGF and human chorionic gonadotropin (hCG) on cell proliferation, steroid hormone and cAMP production in cultured rat ovarian granulosa cells Impact. RESULTS: EGF inhibited hCG-stimulated progesterone production in a dose-dependent manner but had no effect on hCG-stimulated estradiol production. EGF enhanced the production of cAMP in hCG-stimulated granulosa cells and increased the production of cAMP Can stimulate the proliferation of granulosa cells, 3H TdR incorporation increases, hCG does not affect this effect of EGF. Tip EGF and hCG on ovarian function both the interaction, but also their respective roles.