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为探讨APN基因启动子区DNA甲基化及m RNA表达与新疆维吾尔族T2DM发生、发展的相关性,文章选择新疆维吾尔族正常个体50例、肥胖个体48例、肥胖伴T2DM个体26例,收集腹部网膜脂肪组织,利用变性高效液相色谱技术检测APN基因启动子区DNA甲基化情况,应用Real-time PCR方法检测APN基因m RNA表达情况。结果显示,APN基因启动子区DNA甲基化阳性率在正常对照(34%)、肥胖(47.9%)及T2DM组(65.4%)逐渐增加,差异具有统计学意义(P<0.05)。Real-time PCR结果显示,正常对照组APN m RNA相对拷贝数(0.7162)显著高于肥胖(0.4244)及T2DM组(0.4093),差异具有统计学意义(P<0.05)。非T2DM个体相关性分析提示,APN m RNA相对拷贝数与空腹血清葡萄糖(Fasting plasma glucose,FPG)、糖化血红蛋白(Glycosylated hemoglobin,Hb A1c)、甘油三酯(Triglyceride,TG)水平显著负相关(P<0.05)。APN基因启动子区DNA甲基化与其m RNA表达负相关,甲基化阳性组相对拷贝数(0.2700)显著低于阴性组(0.7870),差异具有统计学意义(P<0.01)。以上结果提示,APN基因启动子区DNA甲基化通过抑制其m RNA表达导致糖脂代谢紊乱,可能参与了新疆维吾尔族肥胖及T2DM的发生、发展过程。
In order to investigate the relationship between DNA methylation and m RNA expression in APN gene promoter region and the occurrence and development of T2DM in Xinjiang Uygur, 50 cases of normal Uighur individuals, 48 obese individuals and 26 obese patients with T2DM were collected. Abdominal omentum adipose tissue. The DNA methylation status of APN gene promoter region was detected by denaturing high performance liquid chromatography. The m RNA expression of APN gene was detected by Real-time PCR. The results showed that the positive rate of DNA methylation in APN gene promoter region was increased in normal control (34%), obesity (47.9%) and T2DM group (65.4%), the difference was statistically significant (P <0.05). Real-time PCR results showed that the relative copy number (0.7162) of APN m RNA in normal control group was significantly higher than that in obesity (0.4244) and T2DM group (0.4093), the difference was statistically significant (P <0.05). Correlation analysis of non-T2DM individuals suggested that the relative copy number of APN m RNA was negatively correlated with fasting plasma glucose (FPG), glycosylated hemoglobin (Hb A1c) and triglyceride (TG) levels (P <0.05). DNA methylation in APN gene promoter region was negatively correlated with m RNA expression. The relative copy number (0.2700) in methylation positive group was significantly lower than that in negative group (0.7870) (P <0.01). The above results suggest that DNA methylation in APN gene promoter may be involved in the occurrence and development of obesity and T2DM in Uighur Xinjiang by inhibiting the expression of m RNA and resulting in disorder of glycolipid metabolism.