论文部分内容阅读
目的探讨钙信号在2,2’,4,4’-四溴联苯醚(2,2’,4,4-’tetrabromodiphenyl ethers,PBDE-47)和2,2’4,4’,5,5’六氯联苯(2,2’,4,4’,5,5-’hexachlorobiphenyl,PCB153)诱导人神经母细胞瘤细胞株SH-SY5Y细胞凋亡中的作用。方法PBDE-47设3个剂量组(1、5、10μmol/L),PCB153设1个剂量组(5μmol/L),DMSO为溶剂对照组,PBDE-47与PCB153单独和联合染毒SH-SY5Y细胞24 h后,检测细胞存活率、胞内钙离子浓度[Ca2+]i、细胞凋亡率和Caspase3、Caspase12、Cytochrome C及死亡相关蛋激酶(DAPK)等钙信号通路相关基因mRNA表达水平。结果与溶剂对照组相比,5、10μmol/LPBDE-47染毒剂量组细胞存活率均显著下降,差异有统计学意义(P<0.05);与对照组相比,1、5、10μmol/L PBDE-47剂量组[Ca2+]i和细胞凋亡率均显著增加(P<0.05)。PCB153可明显增加PBDE-47诱导的[Ca2+]i和细胞凋亡率,并存在交互作用(F=6.642,P<0.01;F=10.226,P<0.01)。与对照组相比,PBDE-47可使上述基因mRNA表达水平均增强(P<0.05)。PBDE-47和PCB153联合对Cytochrome C和Caspase3 mRNA表达水平的影响均存在交互作用(P<0.05)。结论钙信号可通过3条经典凋亡通路参与PBDE-47诱导SH-SY5Y细胞的凋亡,PBDE-47和PCB153在诱导SH-SY5Y细胞凋亡过程中存在交互作用。
Objective To investigate the effect of calcium signal on the expression of calcium in 2,2 ’, 4,4’-tetrabromodiphenyl ethers (PBDE-47) and 2,2’4,4’ 5 ’hexachlorobiphenyl (2,2’, 4,4 ’, 5,5’hexachlorobiphenyl, PCB153) induces apoptosis in human neuroblastoma SH-SY5Y cells. Methods PBDE-47 was treated with 1, 5 and 10 μmol / L of PCB153 and 5 μmol / L of PCB153, and DMSO was used as solvent control. PBDE-47 and PCB153 alone and in combination with SH-SY5Y The cell survival rate, intracellular calcium concentration [Ca2 +] i, apoptosis rate and mRNA expression of calcium signaling pathway related genes such as Caspase3, Caspase12, Cytochrome C and DAPK were detected 24 h later. Results Compared with the solvent control group, the cell viabilities of 5, 10μmol / LPBDE-47 treated groups were significantly decreased (P <0.05). Compared with the control group, the cell viabilities of 1,5,10μmol / L PBDE-47 dose group [Ca2 +] i and apoptosis rate were significantly increased (P <0.05). PCB153 could significantly increase [Ca2 +] i and apoptosis rate induced by PBDE-47, and there was interaction (F = 6.642, P <0.01; F = 10.226, P <0.01). Compared with the control group, PBDE-47 mRNA expression levels were increased (P <0.05). The interaction between PBDE-47 and PCB153 on the expression of Cytochrome C and Caspase3 mRNA both had interaction (P <0.05). Conclusions Calcium signaling can participate in the apoptosis of SH-SY5Y cells induced by PBDE-47 through three classical apoptotic pathways. There is interaction between PBDE-47 and PCB153 in inducing the apoptosis of SH-SY5Y cells.