论文部分内容阅读
利用SRAP分子标记方法对伊犁河谷14个野生杏种群,212份种质资源的遗传多样性进行了研究。结果表明,12对SRAP引物共扩增出条带143条,其中122条具有多态性,多态性比率为85.31%。伊犁河谷野生杏仍然维持较高的遗传多样性水平(h=0.2411,I=0.3708,PPB=85.31%),吐尔根乡种群遗传多样性指数最高。伊犁河谷野生杏存在较高水平的种群内遗传变异(76.42%)和较低水平的种群间遗传变异,种群间存在温和稳健的基因流(Nm=1.3680)。UPGMA系统聚类和遗传结构分析均显示,伊犁河谷野生杏可划分为以霍城样本为主的类群Ⅰ,以巩留和伊宁样本为主的类群Ⅱ和以新源样本为主的类群Ⅲ,种群间遗传距离和地理距离存在具有统计学意义的相关性(r=0.2634,P<0.05)。
Genetic diversity of 14 wild apricot populations and 212 germplasm resources in the Ili Valley was studied by SRAP molecular markers. The results showed that a total of 143 bands were amplified with 12 pairs of SRAP primers, of which 122 were polymorphic and the percentage of polymorphic bands was 85.31%. Wild apricot still maintained high genetic diversity in the Ili Valley (h = 0.2411, I = 0.3708, PPB = 85.31%), and Turgen population had the highest genetic diversity index. There was a high level of intraspecific genetic variation (76.42%) and a lower level of inter-population genetic variation in wild Irises in the Ili Valley, with a mild and steady gene flow (Nm = 1.3680) among the populations. UPGMA phylogenetic analysis and cluster analysis showed that the wild apricot in Ili Valley can be divided into three groups, mainly Ⅰ in Huocheng sample, Ⅱ mainly in Gongliu and Yining samples, and Ⅲ mainly in new source samples There was a statistically significant correlation between genetic distance and geographical distance (r = 0.2634, P <0.05).