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已提出,在促性腺激素调节颗粒细胞的甾体生成中至少存在两个依赖于钙的部位。钙调制素(Camodulin)是一种热稳定的钙结合蛋白,分子量约为17,000道尔顿。已证明在牛的颗粒细胞中存在着钙调制素。至今对于在卵巢甾体生成调节中钙调制素的作用尚未弄清。本文使用各种药物以期阐明在卵泡刺激素(FSH)调节的颗粒细胞甾体生成中,钙-钙调制素系统的可能作用。使用未成年雌性大鼠,从24～29±1天,每天腹腔注射一次17β-雌二醇以引起卵泡发育。于第30天将动物处死,立即取下卵巢并放入以Eagle为主的培养环境中。反复穿刺卵泡以放出颗粒细胞,
It has been proposed that there are at least two calcium-dependent sites in steroidogenesis of gonadotropin-regulated granulosa cells. Camodulin is a thermostable calcium-binding protein with a molecular weight of about 17,000 daltons. It has been demonstrated that calmodulin is present in bovine granulosa cells. To date, the role of calmodulin in the regulation of ovarian steroidogenesis has not been clarified. Various drugs are used herein to elucidate the possible role of the calcium-calmodulin system in follicular-stimulating hormone (FSH) -mediated granulosagenesis. Using juvenile female rats, 17β-estradiol was injected intraperitoneally once daily from 24 to 29 ± 1 days to induce follicular development. The animals were sacrificed on the 30th day and the ovaries removed immediately and placed in a culture environment dominated by Eagle. Repeated puncture follicles to release granulosa cells,