采用石蜡切片法,对蛇足石杉的不同器官进行了解剖学特征研究;结合冰冻切片法,用碘化铋钾、氯化铁染色法分别对蛇足石杉的根、茎和叶中的生物碱和黄酮类化合物进行了组织化学定位.结果表明:生物碱主要存在于蛇足石杉根和茎的中柱木质部和皮层的薄壁细胞中,少数存在于叶肉细胞及其胞间隙中,其含量大小顺序为:茎≈根>叶;黄酮类化合物主要存在于蛇足石杉的小型叶肉细胞及其胞间隙中,部分存在于根和茎的中柱韧皮部,含量大小顺序为:叶>茎≈根;在不同的器官中,生物碱和黄酮类化合物的含量存在差异,其中茎和根的生物碱含量较高,而叶中黄酮类化合物含量较高. The anatomical characteristics of different organs of Huperzia serrata were studied by paraffin section method. Combined with frozen section method, the contents of alkaloids in roots, stems and leaves of Huperzia serrata were determined by using potassium bismuth potassium iodide and ferric chloride staining respectively. And flavonoids were studied.The results showed that alkaloids were mainly found in the xylem and cortex pistillate roots and stems of Pistia stratiotes, and a few were found in mesophyll cells and their intercellular space, The order was: stem ≈ root> leaf. Flavonoids were mainly found in the small mesophyll cells and interstitial space of Hippedestrum chinensis, partly in the middle column phloem of roots and stems. The order of content was leaf> stem ≈ root; In different organs, the content of alkaloids and flavonoids varied. The content of alkaloids in stems and roots was higher, and the content of flavonoids in leaves was higher.