论文部分内容阅读
本文应用抗粒细胞集落刺激因子（G─CSF）的单克隆抗体、采用间接免疫吸附法对148例足月新生儿脐血清中G─CSF水平进行了测定，并与41例正常小儿及50例成人血清相比较，同时观察了G─CSF水平与其性别、出生体重、白细胞总数、中性粒细胞绝对计数的关系。结果显示11例（7．4％）脐血清G─CSF水平低于0．5ng／ml，137例（89％）G─CSF均高于0．5ng／ml，其平均值为3．4ng士0.05ng／ml，而且G─CSF水平与其性别、出生体重、白细胞总数及中性粒细胞绝对计数无明显相关（P均＞0.5）；结果还显示1～5个月小儿（41例）及成人血清（50例）G─CSF水平皆低于0．5ng／ml，提示足月新生儿脐血清G─CSF水平大大高于正常小儿及成人水平，本文首次报道中国足月新生儿脐血清G─CSF正常值，为研究新生儿免疫功能及为脐血的合理应用提供了依据。
In this paper, monoclonal antibody against granulocyte colony-stimulating factor (G ─CSF) was used to determine the level of G-CSF in 148 cases of full-term newborn umbilical serum by indirect immunosorbent assay, and compared with 41 cases of normal children and 50 cases Compared with adult serum, the relationship between G-CSF level and sex, birth weight, total white blood cells and absolute neutrophil count were also observed. The results showed that the levels of G-CSF in umbilical serum were lower than 0.5ng / ml in 11 patients (7.4%) and G-CSF was higher than 0.5ng / mL in 137 patients (89%), with an average of 3.4ng 0.05ng / ml. There was no significant correlation between G-CSF level and sex, birth weight, white blood cell count and absolute neutrophil count (all P> 0.5). The results also showed that children aged from 1 to 5 (41) and adults Serum (50 cases) G-CSF levels were lower than 0.5ng / ml, suggesting full-term neonatal umbilical serum G-CSF levels were significantly higher than normal children and adults, the first report of China full-term neonatal umbilical serum G- CSF normal value for the study of neonatal immune function and provide a basis for the rational application of cord blood.