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目的探讨散发性预激综合征与PRKAG2基因多态性的关系。方法散发性预激综合征患者和健康受试者各65例,提取外周血白细胞基因组DNA,聚合酶链反应扩增PRKAG2基因片段,限制性片段长度多态性技术及DNA片段测序法检测基因多态性。结果测序结果与NCBI Genebank BLAST软件进行对比,未发现突变点。结论散发性预激综合征与PRKAG2基因多态性无关。
Objective To investigate the relationship between sporadic WPW syndrome and PRKAG2 gene polymorphism. Methods A total of 65 patients with sporadic WPW syndrome and healthy subjects were enrolled in this study. The genomic DNA of peripheral leukocytes was extracted. The PRKAG2 gene was amplified by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) State. Results The sequencing results were compared with NCBI Genebank BLAST software. No mutation was found. Conclusions Sporadic WPW syndrome is not associated with PRKAG2 gene polymorphism.