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目的 研究家蝇幼虫免疫血淋巴对SMMC 772 1细胞的作用机制。方法 提取家蝇幼虫免疫血淋巴 ,电镜观察对SMMC 772 1细胞超微结构的影响 ,流式细胞仪测定增殖周期的变化。结果 免疫血淋巴作用SMMC 772 1细胞后 ,细胞膜上出现小孔洞、线粒体水肿、粗面内质网轻度扩张、游离的核糖体减少 ;细胞的增殖指数下降 (P <0 .0 1)。结论 家蝇免疫血淋巴作用SMMC 772 1细胞后 ,引起细胞膜通透性增高 ,抑制能量代谢 ,抑制分裂增殖。
Objective To study the mechanism of immune hemolymph of Musca domestica larvae on SMMC 772 1 cells. Methods The immune system of Musca domestica larvae was extracted and the ultrastructure of SMMC 772 1 cells was observed under electron microscope. The proliferation of SMMC 772 1 cells was detected by flow cytometry. Results After SMMC 772 1 cells were immunized with haemolymph, small holes and mitochondria edema were found on the cell membrane. The rough endoplasmic reticulum was slightly expanded and the free ribosomes were decreased. The proliferation index decreased (P <0.01). Conclusion Mus musculus immune haemolymph can induce cell membrane permeability increase, inhibit energy metabolism and inhibit the proliferation of SMMC 772 1 cells.