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试验拟在人工模拟的高甘草酸含量环境下,探寻甘草中次生代谢产物间的相互影响关系,筛选出与甘草酸含量明显相关的次生代谢产物。通过根部浸泡甘草酸铵溶液,分析浸泡后72 h内甘草根中4种次生代谢产物含量的变化,统计学软件分析各成分与甘草酸含量的相关性。结果表明高浓度1.0 mmol·L~(-1)甘草酸的根部浸泡对于在甘草中模拟高甘草酸含量环境是切实可行的,且在短时外源甘草酸刺激时,甘草中甘草酸和甘草苷的含量存在明显的正相关关系。外源甘草酸刺激对甘草植株体内甘草酸的合成积累产生一定的影响,且甘草酸的积累与甘草苷含量密切相关。
In the artificial simulated high glycyrrhizic acid content environment, the relationship between secondary metabolites in Glycyrrhiza uralensis Fisch. Was explored and the secondary metabolites which were obviously related to glycyrrhizic acid content were screened out. The contents of four secondary metabolites in licorice root were analyzed by immersing the solution of ammonium glycyrrhizinate in the roots for 72 h. The correlation between each component and content of glycyrrhizin was analyzed by statistical software. The results showed that root immersion with high concentration of 1.0 mmol·L -1 glycyrrhizic acid was practicable for simulating the environment of high glycyrrhizin content in licorice, and when glycyrrhizic acid was stimulated in a short time, glycyrrhizin and licorice Glycoside content there is a clear positive correlation. Glycyrrhizic acid exogenous glycyrrhizic acid plant glycyrrhizic acid accumulation in the synthesis have a certain impact, and the accumulation of glycyrrhizin and liquiritin content are closely related.