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目的 研究基质金属蛋白酶 (matrixmetalloproteinases,MMPs) 2、9及其组织抑制因子(tissueinhibitorofmetalloproteinases ,TIMPs) 1、2在子宫内膜癌中的表达 ,探讨其与子宫内膜癌浸润转移的关系。方法 应用链霉菌抗生物素蛋白 过氧化物酶免疫组织化学方法和明胶酶谱法对 37例内膜癌及 7例绝经期妇女子宫内膜组织中MMP 2、MMP 9、TIMP 1、TIMP 2蛋白及其活性进行检测。结果 MMP 2、MMP 9及TIMP 1、TIMP 2蛋白主要分布在内膜癌细胞、血管内皮细胞及绝经期子宫内膜腺上皮细胞中 ,在间质细胞中也有少量表达。内膜癌细胞中 ,MMP 2、MMP 9及TIMP 1蛋白的表达 ,病理分级为G3内膜癌的强阳性率分别为 73%、2 0 %及 6 7% ,高于G2 (13%、0及 2 7% )、G1 者 (均为 0 ,P<0 0 5 ) ;深肌层浸润内膜癌的强阳性率分别为 6 3%、16 %及 6 8% ,高于浅肌层浸润的 8%、0及 0 (P<0 0 1) ;有淋巴结转移者的强阳性率分别为 4例中 4例、4例中 3例及 4例中 4例 ,高于无淋巴结转移者的 2 5 %、0及 2 5 % (P <0 0 5 ) ;手术病理分期为Ⅲ～Ⅳ期者强阳性率分别为 5例中 5例、5例中 3例及 5例中 5例 ,高于Ⅰ～Ⅱ期者的 30 %、0及 30 % (P <0 0 5 ) ;TIMP 2蛋白在不同病理分级、肌层浸润、淋巴结转移和手术病理分期的内膜癌细?
Objective To investigate the expression of matrix metalloproteinases 2 and 9 and their tissue inhibitors of metalloproteinases 1 and 2 in endometrial carcinoma and their relationship with invasion and metastasis of endometrial carcinoma. Methods Streptomyces avidin peroxidase immunohistochemistry and gelatin zymography were used to detect the expression of MMP 2, MMP 9, TIMP 1, TIMP 2 in 37 cases of endometrial carcinoma and 7 cases of menopause women And its activity was tested. Results MMP 2, MMP 9, TIMP 1 and TIMP 2 were mainly expressed in endometrial carcinoma cells, vascular endothelial cells and menopausal endometrial glandular epithelial cells, but also expressed in interstitial cells. The positive rates of MMP 2, MMP 9 and TIMP-1 expression in endometrial carcinoma were 73%, 20% and 67%, respectively, which were higher than those in G2 (13%, 0 And 27% respectively), G1 (all 0, P <0 05). The strong positive rates of deep myometrial invasion were 63%, 16% and 68%, respectively, which were higher than those in superficial myometrial invasion (P <0.01). The positive rates of lymph node metastasis were 4 of 4 cases, 3 of 4 cases and 4 of 4 cases, respectively, which were significantly higher than those without lymph node metastasis (P <0.05). The strong positive rates of stage Ⅲ ~ Ⅳ were 5 of 5 cases, 3 of 5 cases and 5 of 5 cases, respectively, with high positive rate 30%, 0%, 30% (P <0 05) of patients with stage Ⅰ ~ Ⅱ; TIMP 2 protein in endometrial cancer with different histological grade, myometrial invasion, lymph node metastasis and surgical pathology