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目的观察地塞米松（ＤＭ）对哮喘豚鼠体外不同密度的嗜酸性粒细胞（Ｅｏｓ）凋亡及ｂｃ－２表达的影响，并探讨激素促进哮喘ＥＯＳ凋亡的机制。方法以卵蛋白激发哮喘豚鼠模型４８ｈ后，进行支气管肺泡灌洗，分离灌洗液中不同密度的Ｅｏｓ。加入ＤＭ（１０～（１０）～１０～５ｍｏｌ／Ｌ），以原位杂交法检测Ｅｏｓ的ｂｃｌ－２表达，ＴＵＮＥＬ法检测Ｅｏｓ的凋亡。结果ＤＭ干预２４ｈ，可见Ｅｏｓ凋亡增加，ｂｃｌ－２ｍＲＮＡ表达降低，且呈剂量依赖性。ｂｃｌ－２ ｍＲＮＡ的表达与Ｅｏｓ凋亡呈负相关。结论ＤＭ可抑制ｂｃｌ－２的表达，促进ＥＯＳ凋亡。ｂｃｌ－２表达的减少可能是ＤＭ调节Ｅｏｓ凋亡的机制之一。
Objective To observe the effect of dexamethasone (DM) on the apoptosis of eosinophils (Eos) and the expression of bc-2 in asthmatic guinea pigs with different densities in vitro. Methods The asthmatic guinea pig model was induced by ovalbumin for 48 hours, and bronchoalveolar lavage was performed to separate Eos with different densities in lavage fluid. DM (10 ~ (10) ~ 10 ~ 5mol / L) was added to detect the expression of bcl-2 of Eos by in situ hybridization. The apoptosis of Eos was detected by TUNEL method. Results DM intervention 24h, Eos apoptosis increased, bcl-2mRNA expression decreased, and in a dose-dependent manner. The expression of bcl-2 mRNA was negatively correlated with Eos apoptosis. Conclusion DM can inhibit the expression of bcl-2 and promote the apoptosis of EOS. The decrease of bcl-2 expression may be one of the mechanisms by which DM regulates Eos apoptosis.