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目的 探讨丙型肝炎病毒 (HCV)包膜基因 E1E2 ,对核心基因 C DNA疫苗诱生的免疫应答有无增强作用 .方法 构建包含HCV C或 CE1E2基因片段的真核表达载体 p HCV- C和 p HCV- CE1E2 ,分别接种于 BAL B/c小鼠股四头肌 (以空载体 pc DNA3作为对照 ) ,每间隔 2 wk加强免疫 1次 .用 EL ISA法检测免疫小鼠血清中抗 HCV C特异性抗体的产生 .以 p HCV- C转染并表达 HCc Ag的 Sp2 /0细胞为靶细胞 ,采用 5 1 Cr释放试验检测特异性 CTL的杀伤作用 .结果 两个实验组免疫的 2 0只小鼠均产生抗 HCV C特异性抗体 ,当效 /靶细胞比例为 10 0∶ 1时 ,CTL 的杀伤率均明显高于对照组 (P<0 .0 1) ;而 p HCV- CE1E2与 p HCV-C组之间 ,无论是抗 HCV C抗体的滴度还是 CTL的杀伤率均无显著性差异 (P>0 .0 5 ) .结论 E1E2基因的加入 ,并没有增加HCV C基因 DNA疫苗诱导的抗 HCc Ag特异性抗体的滴度和 CTL 的杀伤作用 .
Objective To investigate whether the hepatitis C virus envelope gene E1E2 enhances the immune response induced by core gene C DNA vaccine.Methods The eukaryotic expression vector p HCV-C and p HCV-CE1E2 were inoculated into the quadriceps femoris of BALB / c mice (empty vector pcDNA3 as control) and boosted once every 2 weeks.The serum levels of anti-HCV C in the immunized mice Antibody production.Cp2 / 0 cells transfected with pCTC-C and expressing HCcAg as target cells were tested for the killing effect of specific CTL by 5 1 Cr release test.Results 20 mice immunized with two experimental groups The anti-HCV C-specific antibodies were all produced in mice. When the ratio of target cells to target cells was 10 0: 1, the killing rate of CTL was significantly higher than that of the control group (P <0.01) There was no significant difference in the titer of anti-HCV C antibody or the killing rate of CTL between group C and group C (P> 0.05) .Conclusion The addition of E1E2 gene did not increase the DNA vaccine induced by HCV C gene Titers of anti-HCc Ag-specific antibodies and cytotoxicity against CTLs.