The endosymbionts play vital roles in growth,development and reproduction in insects.Yeast-like endosymbionts (YLSs) have been well studied in Nilaparvata lugens (N.lugens),but little is known about the tissue-specific bacterial microbiomes,especially on the microbial intersection among intal tissues.Here,the correlation of microbial composition,structure,dispersal ability and functional profiling were illuminated in two tissues,the fat body and ovary in N.lugens.A total of 11 phyla and 105 genera were captured from all samples;Firmicutes and Proteobacteria were the most predominant and accounted for more than 99％ in all samples.However,the relative abundance of Firmicutes and Proteobacteria was significantly different in ovary and fat body through Fisher’s Least Significant Difference test.Microbial diversity but not the richness index in the two tissues exhibited significant difference.Furthermore,the microbial community structure of the ovary and fat body were primarily determined by tissue quality.Firmicutes showed strong dispersal ability between ovary and fat body based on the quantitative null model assessing,indicating the frequent interaction of these microbiomes in the two tissues.In addition,the Kyoto Encyclopedia of Genes and Genomes pathways of microbial participation were delineated.The ten most abundant pathways counted for over 46％ of the annotation and were shared between the two tissues,mainly containing Energy Metabolism and Amino Acid Metabolism/Biosynthesis.The results will provide insights into the correlation of microbial community structttre between ovary and fat body ofN.lugens.