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AIM:To investigate the multiple gene differential expressionpatterns in human ischemic liver and to produce the evidenceabout the hepatic ischemic safety time.METHODS:The responses of cells to hepatic ischemia andhypoxia at hepatic ischemia were analyzed by cDNAmicroarrary representing 4000 different human genescontaining 200 apoptotic correlative genes.RESULTS:There were lower or normal expression levelsof apoptotic correlative genes during the periods of hepaticischemia for 0-15 min,the maintenance homostatic geneswere expressed significantly higher at the same time.Butat the hepatic ischemia for 30 min,the expression levels ofmaintenance homeostatic genes were down-regulated,theexpressions of many apoptotic correlative genes and nucleartranscription factors were activated and up-regulated.CONCLUSION:HIF-1,APAF-1,PCDC10,FBX5,DFF40,DFFAXIAP,survivin may be regarded as the signal genes to judgethe degree of hepatic ischemic-hypoxic injure,and theapoptotic liver cell injury due to ischemia in different timelimits.The safe limit of human hepatic warm ischemic timeappears to be generally less then 30 min.
A investigate: investigate the multiple gene differential expression patterns in human ischemic liver and to produce the evidenceabout the hepatic ischemic safety time. METHODS: The responses of cells to hepatic ischemia and hypoxia at hepatic ischemia analyzed by cDNA microarrays representing 4000 different human genes associated to 200 apoptotic correlative genes .RESULTS: There were lower or normal expression levels of apoptotic correlative genes during the periods of hepaticischemia for 0-15 min, the maintenance homostatic genes were highly significant at the same time. Butat the hepatic ischemia for 30 min, the expression levels of maintenance home homeostatic genes were down-regulated, theexpressions of many apoptotic correlative genes and nucleartranscription factors were activated and up-regulated.CONCLUSION: HIF-1, APAF-1, PCDC10, FBX5, DFF40, DFFAXIAP, survivin may be regarded as the signal genes to judgethe degree of hepatic ischemic-hypoxic injure, and the apoptotic liver cell injury due to ische mia in different timelimits. The safe limit of human hepatic warm ischemic time needs to be less less then 30 min.