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目的探讨CD44v16与可溶性CD44v6(sCD44v6)在胃癌中的表达及与胃癌生物学行为和预后的关系。方法应用免疫组化S-P法检测103例胃癌组织和10例正常胃黏膜标本的CD44v6表达,并对患者进行术后随访,随访期为3-91个月;应用酶联免疫吸附(ELISA)法检测86例胃癌、30例胃溃疡患者和30例健康人外周血血清中sCD44v6的浓度,并对患者进行术后随访,随访期为1～91个月。结果(1)CD44v6在正常胃黏膜中不表达,而在胃癌中的表达率为60.2%;CD44v6的表达与淋巴结转移、TNM分期、脉管是否存在癌栓以及Bomnann分型有关(P<0.05),而与胃癌浸润深度、是否远处转移、分化程度以及患者的生存率无关(P>0.05);9例伴有肝转移的胃癌患者中,有7例CD44v6为强阳性表达。(2)胃癌患者外周血血清中sCD44v6的浓度明显高于胃溃疡组和健康对照组(P<0.01);胃癌根治术后患者血液中sCD44v6的浓度比术前明显下降(P<0.01),而姑息术后患者的浓度变化却不明显(P>0.05);sCIM4v6的浓度与胃癌浸润深度、淋巴结转移、是否远处转移、脉管是否存在癌栓、Borrmann分型以及分化程度无关;sCD44v6浓度较高组的生存率较sCD44v6浓度较低组高,且差异有统计学意义(P=0.0281),但经Cox回归分析显示,sCD44v6的浓度与生存期有关(P=0.415),而手术方式却与生存期有关(P=0.000)。(3)胃癌组织中CD44v6的表达与外周血血清中sCD44v6的表达无明显平行关系(P>0.05)。结论CD44v6的表达对评估胃癌生物学行为有参考价值,但是否与预后有关仍需探讨;sCD44v6的表达对胃癌辅助诊断、手术彻底性判定及生物学行为评估有一定的意义;CD44v6与sCD44v6的表达无明显平行关系。
Objective To investigate the relationship between the expression of CD44v16 and soluble CD44v6 (sCD44v6) in gastric cancer and their biological behavior and prognosis. Methods The expression of CD44v6 in 103 gastric cancer tissues and 10 normal gastric mucosa specimens was detected by immunohistochemical SP method. The patients were followed up for 3-91 months. The levels of CD44v6 were detected by enzyme linked immunosorbent assay (ELISA) 86 cases of gastric cancer, 30 cases of gastric ulcer patients and 30 healthy persons serum sCD44v6 concentration, and patients were followed up for a follow-up of 1-91 months. Results (1) CD44v6 was not expressed in normal gastric mucosa but 60.2% in gastric carcinoma. The expression of CD44v6 was correlated with lymph node metastasis, TNM stage, presence of tumor thrombus and Bomnann classification (P <0.05) , But not with depth of invasion, distant metastasis, differentiation and the survival rate of patients (P> 0.05). Of the 9 patients with hepatic metastases, 7 were strongly positive for CD44v6. (2) The concentration of sCD44v6 in peripheral blood of gastric cancer patients was significantly higher than that in gastric ulcer patients and healthy controls (P <0.01). The concentration of sCD44v6 in patients with gastric cancer was significantly lower than that before operation (P <0.01) The concentration of sCIM4v6 was not related to the depth of invasion of gastric cancer, lymph node metastasis, distant metastasis, tumor embolism, Borrmann classification and the degree of differentiation. The concentration of sCD44v6 was higher than that of sCD44v6 The survival rate of high group was higher than that of lower sCD44v6 group (P = 0.0281). However, the Cox regression analysis showed that the concentration of sCD44v6 was associated with survival (P = 0.415) Survival related (P = 0.000). (3) There was no significant correlation between the expression of CD44v6 and the expression of sCD44v6 in peripheral blood (P> 0.05). Conclusions The expression of CD44v6 is valuable in assessing the biological behavior of gastric cancer. However, whether it is related to the prognosis remains to be explored. The expression of sCD44v6 is of significance for adjuvant diagnosis of gastric cancer, completeness of surgery and assessment of biological behavior. The expressions of CD44v6 and sCD44v6 No obvious parallel relationship.