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目的探讨绞股蓝不同成分(绞股蓝总皂苷Gps、绞股蓝皂苷XILX GpXILX、绞股蓝皂苷A GpA、人参皂苷GRb3)对氧化低密度脂蛋白诱导内皮细胞氧化应激损伤线粒体膜电位的影响。方法采用CCK-8法分别观察不同浓度Gps、GpXILX、GpA、GRb3对EA.Hy926细胞活力的影响,明确它们作用于EA.Hy926细胞的浓度;采用ox-LDL诱导氧化应激损伤模型,分别予Gps、GpXILX、GpA、GRb3处理24h。用微量法检测细胞氧化应激损伤丙二醛(MDA)的含量、超氧化物歧化酶(SOD)的活性及T-AOC含量,流式细胞仪检测各组细胞线粒体膜电位的变化。结果不同浓度Gps、GpXILX、GpA、GRb3对EA.hy926细胞增殖无明显的抑制效应。ox-LDL诱导细胞后使细胞中MDA含量增高,SOD活力降低,T-AOC含量降低。而Gps、GpXILX、GpA、GRb3预处理能降低MDA的含量,Gps、GpXILX、GRb3能增强SOD的活性,提高T-AOC含量。线粒体膜电位数据显示,ox-LDL诱导细胞后使细胞线粒体膜电位降低。而Gps、GpXILX、GpA、GRb3处理细胞后能显著增加EA.hy926细胞线粒体膜电位。结论不同成分绞股蓝对ox-LDL所致内皮细胞氧化应激损伤具有不同程度的保护作用,可能与降低EA.hy926细胞氧化应激,提高抗氧化防御和稳定线粒体膜电位有关。
Objective To investigate the effects of different components of Gynostemma pentaphyllum (Gps, GpXILX, GpA, GRb3) on mitochondrial membrane potential of oxidative stress induced by oxidative low density lipoprotein in endothelial cells. Methods The effects of Gps, GpXILX, GpA and GRb3 at different concentrations on the viability of EA.Hy926 cells were observed by CCK-8 assay to determine their effect on EA.Hy926 cells. Ox-LDL-induced oxidative stress injury was induced by Gps, GpXILX, GpA, GRb3 for 24 h. The content of malondialdehyde (MDA), activity of superoxide dismutase (SOD) and content of T-AOC in cell oxidative stress injury were detected by micro-assay. The changes of mitochondrial membrane potential were detected by flow cytometry. Results Different concentrations of Gps, GpXILX, GpA and GRb3 had no obvious inhibitory effect on the proliferation of EA.hy926 cells. Ox-LDL induced cell MDA content increased, SOD activity decreased, T-AOC content decreased. Gps, GpXILX, GpA, GRb3 pretreatment can reduce the content of MDA, Gps, GpXILX, GRb3 can enhance the activity of SOD and increase the content of T-AOC. Mitochondrial membrane potential data showed that ox-LDL induced cell mitochondrial membrane potential decreased. Gps, GpXILX, GpA and GRb3 could significantly increase the mitochondrial membrane potential of EA.hy926 cells. Conclusion Gynostemma pentaphyllum may have different protective effects on oxidative stress induced by ox-LDL in endothelial cells, which may be related to the reduction of oxidative stress, antioxidative defense and mitochondrial membrane potential in EA.hy926 cells.