目的探讨学龄前儿童血清谷草转氨酶(AST)正常参考值与现有临床标准的差异,旨在为临床重新制定学龄前儿童血清AST参考标准提供一定的思路。方法选取2015年1月至2015年12月在东方市八所镇、感城镇、板桥镇筛选出720名(男、女各360例)1-7周岁健康学龄前儿童作为受试对象。所有受试对象在体检时空腹,使用抗凝管收集肘静脉血2m L,非抗凝管收集肘静脉血3 ml进行检测。检测患者血清AST水平和乙肝两对半、丙肝、梅毒、HIV结果,所有检测中出现任何阳性结果均将数据剔除。同时由防保科医生对受试对象进行体格检查,剔除不合格的数据。分别统计分析不同性别、不同年龄以及不同地区学龄前儿童血清AST的水平差异;若差异有显著性,则分别建立不同的正常参考值;若没有显著性差异则统一建立正常的参考值。结果血清AST均值为诶35.0724 U/L,最大值为87.00 U/L,最小值为7.30 U/L,根据计算得到的95%置信区间为14.50~55.64 U/L,高于现有参考标准。性别与血清AST水平的结果表明,学龄前儿童性别与血清AST之间的差异没有显著性(t=1.42,P>0.05)。不同年龄段之间有显著性差异,其中1~3岁、4岁和5岁样本之间没有显著性差异,而6~7岁与1~3岁、4岁和5岁之间均有显著性差异(P<0.05)。地区影响的分析结果表明,不同地区的学龄前儿童平均血清AST没有显著性差异(P>0.05)。结论 6~7岁的学龄前儿童血清AST水平显著低于其他年龄段的血清AST水平,值得临床重视。制定不同地区,不同年龄段的学龄前儿童AST水平参考标准具有重要的意义。 Objective To explore the difference between serum reference value of aspartate aminotransferase (AST) in preschool children and the existing clinical standards, and to provide some ideas for revising the reference standard of serum AST in preschool children. Methods From January 2015 to December 2015, 720 healthy preschool children aged 1-7 years (360 boys and 360 girls) were screened out in Bachu, Yicheng and Banqiao townships in Dongfang city as subjects. All subjects were fasting on physical examination, 2 mL of elbow venous blood was collected using an anticoagulant tube, and 3 mL of elbow venous blood was collected from a non-anticoagulant tube. Patients were tested for serum AST levels and two pairs of hepatitis B, hepatitis C, syphilis, HIV results, and any positive results from all tests were excluded. At the same time by the prevention physicians on the subjects of physical examination, excluding unqualified data. Statistical analysis of different gender, age and different regions of preschool children serum AST levels differences; if the difference was significant, respectively, to establish different normal reference values; if no significant difference, the uniform establishment of a normal reference value. Results The mean serum AST was 35.0724 U / L, the maximum was 87.00 U / L and the minimum was 7.30 U / L. The calculated 95% confidence interval was 14.50-55.64 U / L, which was higher than the existing reference standard. Results of sex and serum AST levels showed no significant difference between preschool children’s gender and serum AST (t = 1.42, P> 0.05). There were significant differences between different age groups, of which 1 to 3 years old, 4 years old and 5 years old no significant difference between the samples, and 6 to 7 years and 1 to 3 years old, 4 years and 5 years old were significant Sex differences (P <0.05). The analysis of regional influence showed that the average serum AST of preschool children in different regions had no significant difference (P> 0.05). Conclusions Serum AST levels in preschool children aged 6-7 years are significantly lower than those in other age groups, which deserves clinical attention. The development of reference standards for AST levels in preschool children in different regions and at different ages is of great significance.