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双光子荧光显微成像技术具有较低的光漂白与光损伤、较佳的成像对比度、较深的穿透深度等优点。采用结扎大鼠冠状动脉左前降支的方法建立急性心肌缺血模型,通过双光子荧光显微成像技术获得不同结扎时间下大鼠左心室前壁心肌组织的双光子荧光图像。从中分析了不同结扎时间下心肌组织的形态学变化,实现对心肌组织损伤范围的确定;并对双光子荧光图像进行快速傅里叶变换(FFT),使用方向指数这一参数对心肌缺血的程度进行初步的量化评估。结果表明,双光子荧光显微成像技术结合FFT分析技术,有望在心肌缺血性疾病的诊断和治疗中实时、快速、准确地判断心肌缺血的范围和评估其损伤程度。
Two-photon fluorescence microscopy has the advantages of lower photobleaching and light damage, better imaging contrast and deeper penetration depth. Acute myocardial ischemia models were established by ligation of the left anterior descending coronary artery in rats. Two-photon fluorescence images of the anterior myocardium in the left ventricle were obtained by two-photon fluorescence microscopy. From the analysis of the morphological changes of myocardial tissue under different ligation time to determine the scope of myocardial damage; and two-photon fluorescence image fast Fourier transform (FFT), the use of the index of this parameter on myocardial ischemia Degree of preliminary quantitative assessment. The results show that the two-photon fluorescence microscopy combined with FFT analysis technology is expected in the diagnosis and treatment of myocardial ischemic disease in real time, quickly and accurately determine the scope of myocardial ischemia and assess the extent of damage.