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在伯氏鼠疟原虫感染的小鼠体内试验中研究了小柴胡汤及其与青蒿素配伍的抗疟作用。单用小柴胡汤醇提取物的抗疟作用甚微，当它和青蒿素联合用药时，二药并无抗疟增效作用，而且对伯氏鼠疟的复燃也无影响。
In vivo studies of mice infected with Plasmodium berghei have studied Xiaochaihu Decoction and its compatibility with artemisinin in the antimalarial effect. The antimalarial effect of Xiaochaihu Tang alcohol extract alone was minimal. When it was combined with artemisinin, the two drugs had no antimalarial synergistic effect, and had no effect on the reactivation of P. berghei.