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目的:探讨环状RNA-UBXN7（circ_UBXN7）对肝癌细胞增殖、迁移以及凋亡的影响，并初步探讨其中的分子机制。方法:采用qRT-PCR检测circ_UBXN7在肝癌组织和细胞中的表达水平，并分析circ_UBXN7与患者临床病理因素如年龄、性别、肿瘤体积、病理分型、分期、淋巴结转移之间的相关性。构建携带circ_UBXN7全长序列的慢病毒（Lenti-circ_UBXN7）和携带circ_UBXN7 shRNA的慢病毒（Lenti-circ_UBXN7-shRNA）分别转染肝癌细胞株（HepG2和HUH-7）。CCK-8实验检测circ_UBXN7表达上调或下调后对HepG2和HUH-7细胞的增殖能力的影响。Annexin V/PI实验检测circ_UBXN7表达上调或下调后HepG2和HUH-7细胞的凋亡变化。JC-1法检测circ_UBXN7表达上调或下调后HepG2和HUH-7细胞线粒体势能的变化。Transwell检测circ_UBXN7表达上调或下调后HepG2和HUH-7细胞迁移能力变化。蛋白质印迹法检测细胞上皮间质化标志蛋白TWIST、E-钙黏蛋白（E-cadherin）、N-cadherin、Vimentin的表达变化。circ_UBXN7表达水平与临床病理因素采用χn 2检验，两组比较采用n t检验，三组及以上比较采用单因素方差分析且组间比较采用LSD法。n 结果:circ_UBXN7在肝癌组织中表达显著高于癌旁组织，且其表达水平与肿瘤体积、分期和淋巴结转移显著正相关（n P < 0.05）。Lenti-circ_UBXN7可以上调HepG2和HUH-7细胞内circ_UBXN7表达并促进细胞增殖；Lenti-circ_UBXN7-shRNA可以下调circ_UBXN7表达并诱导细胞凋亡。Lenti-circ_UBXN7-shRNA可以导致细胞线粒体膜势能降低。Lenti-circ_UBXN7可以促进细胞迁移，而Lenti-circ_UBXN7-shRNA可以抑制细胞迁移。Lenti-circ_UBXN7可以诱导Twist、N-cadherin、Vimentin蛋白表达增高，并降低E-cadherin蛋白表达；而Lenti-circ_UBXN7-shRNA对各蛋白表达水平的影响则相反。Starbase V2.0软件显示miR-203a与circ_UBXN7存在潜在结合位点，并且在HepG2和HUH-7细胞中miR-203a与circ_UBXN7表达水平呈负相关。n 结论:circ_UBXN7在肝癌发生发展中发挥重要促癌作用，有望成为肝癌治疗的潜在靶点。“,”Objective:To investigate the effect and molecular mechanism of circular RNA-UBXN7 (circ_UBXN7) on the proliferation, migration and apoptosis of hepatocellular carcinoma cells.Methods:Circ_UBXN7 expression in the tissues and cells of hepatocellular cancer was detected by quantitative real-time polymerase chain reaction (qRT-PCR), and the relationship between circ_UBXN7 expression and clinicopathological features, including age, gender, tumor volume, pathological classification, staging, and lymph node metastasis was analyzed. The full-length sequence of circ_UBXN7 with lentivirus carrying lenti circ_UBXN7 and lenti circ_UBXN7 shRNA was constructed to transfect hepatocellular cell lines (HepG2 and Huh-7), respectively. CCK-8 experiments were performed to detect the ability of up- or down-regulation of circ_UBXN7 on the proliferation of HEPG2 and HUH-7 cells. Annexin V / PI experiment was used to detect the changes in apoptosis of HEPG2 and HUH-7 cells after up-regulation or down-regulation of circ_UBXN7 expression. JC-1 assay was used to detect the changes in mitochondrial potential energy of HEPG2 and HUH-7 cells after up-regulation or down-regulation of circ_UBXN7 expression. Transwell was used to detect the migration ability of HEPG2 and HUH-7 cells after up-regulation or down-regulation of circ_UBXN7 expression. Western blotting was used to detect the expressional change of TWIST, E-cadherin, N-cadherin and vimentin. Statistical analysis: The expression levels of circ_UBXN7 and clinicopathological features were measured by chi-square test. Two groups were compared by t-test and three groups and above were compared by single factor analysis of variance. LSD method was used for comparison between groups.Results:The expression of circ_UBXN7 in liver cancer tissues was significantly higher than adjacent tissues, and its expression level was significantly positively correlated with tumor volume, stage, and lymph node metastasis (n P < 0.05). Lenti-circ_UBXN7 had up-regulated the expression of circ_UBXN7 in HEPG2 and HUH-7 cells and promoted cell proliferation. Lenti-circ_UBXN7-shRNA had down-regulated the expression of circ_UBXN7 and induced apoptosis. Lenti-circ_UBXN7-shRNA had reduced the mitochondrial membrane potential of cells. Lenti-circ_UBXN7 had promoted cell migration, while lenti-circ_UBXN7-shRNA had inhibited cell migration. Lenti-circ_UBXN7 had induced increased expression of Twist, N-cadherin, and Vimentin proteins, and reduced the expression of E-cadherin protein. Lenti-circ_UBXN7-shRNA had opposite effects on the expression levels of each protein. Starbase V2.0 software showed that miR-203a and circ_UBXN7 had potential binding sites, and miR-203a and circ_UBXN7 expression levels were negatively correlated in HEP ??G2 and HUH-7 cells.n Conclusion:circ_UBXN7 plays an important role in promoting the occurrence and development of liver cancer, and is expected to become a potential target for the treatment of liver cancer.