为了考察中草药添加剂对公猪精液品质、血清激素及基因表达的影响,试验将24头日龄、体况相近的大约克种公猪随机分为3组,对照组饲喂基础日粮,试验1组饲喂基础日粮+2%中草药添加剂,试验2组饲喂基础日粮+3%中草药添加剂,测定公猪精液量、精子畸形率、精液密度、精子活力、促卵泡生成素(FSH)、促黄体生成素(LH)和睾酮(T)含量以及公猪雄性相关基因(GAPDH、CREB、CYP19)表达量等相关数据。结果表明:与对照组相比,试验1,2组精液量有所提高;试验1,2组精子畸形率均低于对照组,其中试验2组显著降低(P<0.05);试验1,2组精子密度、精子活力高于对照组,其中试验2组显著高于对照组(P<0.05)。与对照组相比,试验1,2组FSH及试验2组LH、T含量显著增加(P<0.05);试验2组CREB基因表达量比对照组上调了2.66倍(P<0.05),CYP19基因表达量上调了4.63倍(P<0.01)。说明饲粮中添加中草药添加剂可以通过调节激素水平改善精液品质,从而提高种公猪精子活力、密度,降低畸形率;3%中草药添加剂效果更佳。 In order to investigate the effect of Chinese herbal medicine additive on boar semen quality, serum hormone and gene expression, 24 male and female boars of similar age and body condition were randomly divided into three groups. The control group was fed basal diet, The rats in group 2 were fed basal diet supplemented with 2% Chinese herbal medicine. The rats in group 2 were fed basal diet supplemented with 3% Chinese herbal medicine. The boar sperm quantity, sperm deformity, sperm density, sperm motility, follicle stimulating hormone (FSH) Luteinizing hormone (LH), testosterone (T) and the expression of male related gene (GAPDH, CREB, CYP19) in boars. The results showed that compared with the control group, the semen volume of group 1 and group 2 increased. The sperm deformity rate of group 1 and group 2 was lower than that of control group, of which group 2 was significantly reduced (P <0.05) Group sperm density, sperm motility than the control group, the experimental group 2 was significantly higher than the control group (P <0.05). Compared with the control group, the contents of LH and T in FSH group and experimental group 2 were significantly increased (P <0.05); the CREB gene expression in experimental group 2 was 2.66 times more than that of the control group (P <0.05); the CYP19 gene The expression level was up-regulated by 4.63 times (P <0.01). The results showed that adding Chinese herbal medicine to diets can improve sperm quality by regulating hormone levels, so as to improve sperm motility and density of boars and reduce deformity rate; 3% Chinese herbal medicine additive is better.