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目的:探讨加减抵挡汤治疗肾小球硬化的机制。方法:采用单侧肾切除重复阿霉素注射造成肾小球硬化大鼠模型,以加减抵挡汤治疗并用西药科素亚为阳性对照,观察模型组和各治疗组的肾功能及24h尿蛋白定量,采用逆转录多聚酶链反应(RT-PCR)测定各组大鼠肾组织TIMP-1及其PAI-1的mRNA的表达。结果:加减抵挡汤各组均能降低肾小球硬化大鼠增高的24h尿蛋白,改善肾功能,且加减抵挡汤能下调大鼠肾组织TIMP-1和PAI-1mRNA的表达。结论:加减抵挡汤通过下调肾小球硬化大鼠肾组织TIMP-1和PAI-1 mRNA的表达,延缓肾功能衰竭。
Objective: To explore the mechanism of treatment of glomerulosclerosis by adding or reducing resisting decoction. METHODS: Unilateral nephrectomy with repeated doxorubicin injections resulted in a glomerulosclerotic rat model. With addition and subtraction of antidiabetic decoction and Western drug Cosmecein as a positive control, kidney function and 24-hour urinary protein were observed in the model group and each treatment group. Quantitatively, mRNA expression of TIMP-1 and its PAI-1 in kidneys of each group was determined by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Addition and subtraction of decoction each group can reduce the increased 24-hour urinary protein in glomerulosclerotic rats and improve renal function, and addition and reduction of decoction can down-regulate the expression of TIMP-1 and PAI-1 mRNA in rat renal tissue. Conclusion: The addition and subtraction of decoction can delay the expression of TIMP-1 and PAI-1 mRNA in renal tissue of rats with glomerulosclerosis and delay the renal failure.