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目的 :观察肾病综合征 (NS)患儿外周血中性粒细胞 (PMN)凋亡 ,并检测外周血中细胞因子IL -8、IL -6、TNF -α、NO水平的变化 ,探讨其对PMN凋亡的影响。方法 :采用流式细胞术检测 2 8例初诊和 2 0例缓解期肾病综合征患儿外周血PMN凋亡水平 ,ELISA法检测细胞因子IL -8、IL -6、TNF -α、NO水平的变化。结果 :活动期肾病综合征患儿PMN凋亡率明显低于对照组和缓解后肾病综合征患儿。缓解组肾病综合征患儿PMN凋亡率与对照组无差别 ;初诊肾病综合征患儿外周血中IL -8、IL -6、TNF -α、NO水平均高于对照组和缓解组 ,且与PMN凋亡呈负相关 ,与病情呈正相关 ;缓解组患者IL -8、IL -6、TNF -α、NO与对照组无显著性差异。结论 :肾病综合征患儿PMN凋亡延迟 ,且与病情及疗效密切相关。各种炎性细胞因子产生过多可能是导致PMN凋亡延迟的重要机制。适度调控PMN凋亡 ,有可能成为治疗肾病综合征的有效途径
Objective: nephrotic syndrome (NS) in peripheral blood neutrophils (PMN) apoptosis, and detecting a change in peripheral blood cytokine IL -8, IL -6, TNF -α, NO and to explore its Effect of PMN apoptosis. Method: detection of apoptosis by flow cytometry using peripheral blood PMN newly diagnosed children 28 and 20 remission of nephrotic syndrome, ELISA assay cytokine IL -8, IL -6, TNF -α, NO Level Variety. Results: The apoptosis rate of PMN in children with active nephrotic syndrome was significantly lower than that in control group and children with nephrotic syndrome after remission. Neutrophilic nephrotic syndrome in children with PMN apoptosis rate was no difference with the control group; newly diagnosed nephrotic syndrome in children with peripheral blood IL -8, IL -6, TNF-α, NO levels were higher than the control group and remission group, and Which was negatively correlated with apoptosis of PMN and positively correlated with the disease. There was no significant difference between IL-8, IL-6, TNF-α and NO in the remission group and the control group. Conclusion: The apoptosis of PMN in children with nephrotic syndrome is delayed, and is closely related to the condition and curative effect. Excessive production of various inflammatory cytokines may be an important mechanism leading to delayed apoptosis of PMN. Moderate regulation of PMN apoptosis may be an effective way to treat nephrotic syndrome