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目的观察白桦脂醇对大鼠酒精性肝损伤的抗氧化作用。方法将大鼠随机分为6组,正常组、低剂量组、中剂量组、高剂组量、阳性对照组(益肝灵组)和模型组。采用白酒灌胃的方法建立大鼠酒精性肝病模式,给予白桦脂醇保护。测定肝匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽-S-转移酶(GST)活性和还原性谷胱甘肽(GSH)、丙二醛(MDA)含量,同时进行肝脏的病理切片观察。结果高剂量白桦脂醇组中SOD、GSH-Px和GST活性和GSH含量明显升高,MDA含量明显降低,和模型组比较,差异均有统计学意义(P<0.01或P<0.05)。阳性对照组可明显提高酒精性肝损伤大鼠肝组织中SOD、GSH-Px、GST活性和GSH含量,明显降低MDA含量,与模型组比较,差异均有统计学意义(P<0.01或P<0.05)。高剂量白桦脂醇还能明显改善由酒精引起的肝组织脂肪样变和坏死。结论白桦脂醇能抑制酒精诱导脂质过氧化反应对肝组织的损伤,对酒精性肝损伤有明显的保护作用。
Objective To observe the antioxidative effects of betulin on alcoholic liver injury in rats. Methods The rats were randomly divided into 6 groups, normal group, low dose group, middle dose group, high dose group, positive control group (Yiganling group) and model group. The alcoholic liver disease model was established by the method of gavage by gavage, and the protection was achieved by betulin. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) and reducing glutathione (GSH) , Malondialdehyde (MDA) content, while liver biopsy. Results The activities of SOD, GSH-Px and GST and the content of GSH in high-dose Betulin group were significantly increased and MDA content was significantly lower than that in model group (P <0.01 or P <0.05). The positive control group could significantly increase the activity of SOD, GSH-Px, GST and the content of GSH in the liver tissue of alcoholic liver injury rats, and significantly reduce the content of MDA. Compared with the model group, the differences were statistically significant (P <0.01 or P < 0.05). High-dose betulin also significantly improved hepatic steatosis and necrosis caused by alcohol. Conclusion Betulin can inhibit alcohol-induced lipid peroxidation on liver tissue injury, alcoholic liver injury has a significant protective effect.