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目的研究干酪乳酸杆菌代谢物对体外白假丝酵母菌生物被膜的抑菌作用及其机制。方法通过萃取、蒸馏的方式浓缩干酪乳酸杆菌代谢物。采用甲基四氮盐(XTT)减低法测定干酪乳酸杆菌代谢物对白假丝酵母菌YEM30游离态及生物被膜态的最小抑菌浓度(MIC80),定量检测干酪乳酸杆菌代谢物对白假丝酵母菌体外代谢活动的影响,观察白假丝酵母菌形态学的转变,并通过双向凝胶电泳(2-DE)对比干酪乳酸杆菌代谢物处理前后白假丝酵母菌蛋白的表达差异。结果干酪乳酸杆菌代谢物对白假丝酵母菌的游离态、生物被膜态的最小抑菌稀释倍数分别为1∶256和1∶128。稀释倍数为1∶256的干酪乳酸杆菌代谢物可以明显干扰白假丝酵母菌酵母相向菌丝相的转化。比较双向电泳图谱上获得6个显著蛋白斑点,对其中的4个进行鉴定,结果显示在干酪乳酸杆菌代谢物的刺激下,烯醇酶、苹果酸脱氢酶、泛素连接酶表达升高,己糖激酶表达下降。结论干酪乳酸杆菌代谢物对体外白假丝酵母菌生物被膜具有抑制作用。
Objective To study the antibacterial activity of Lactobacillus casei metabolites on the biofilm of Candida albicans in vitro and its mechanism. Methods Lactobacillus casei metabolites were concentrated by extraction and distillation. The minimum inhibitory concentration (MIC80) of Lactobacillus casei metabolites on the free and biofilm state of Candida albicans YEM30 was determined by methyltetrazolium salt (XTT) reduction method. The metabolites of Lactobacillus casei against Candida albicans The metabolic changes of Candida albicans were observed. The two Candida albicans proteins were analyzed by two-dimensional gel electrophoresis (2-DE). Results The minimum antibacterial dilutions of Lactobacillus casei metabolites against Candida albicans were 1:256 and 1:128, respectively. Lactobacillus casei metabolites with a dilution factor of 1:256 can obviously interfere with the transformation of Candida albicans yeast to mycelial phase. Comparison of two-dimensional electrophoresis patterns obtained six significant protein spots, of which four were identified, the results showed that Lactobacillus cheese lactobacillus metabolites, enolase, malate dehydrogenase, ubiquitin ligase expression increased, Hexokinase expression decreased. Conclusion Lactobacillus casei metabolites have an inhibitory effect on Candida albicans biofilm in vitro.