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目的：探讨应用流式细胞仪检测恶性肿瘤患者外周血活化Ｔ淋巴细胞含量及该参数对于恶性肿瘤诊断的临床价值。方法：应用流式细胞免疫学方法，对１２种共１１５例恶性肿瘤患者外周血活化Ｔ细胞进行了定量测定。结果：恶性肿瘤外周血活化Ｔ细胞含量明显高于正常对照组活化Ｔ细胞的含量（Ｐ＜０．０５）。结论：活化Ｔ淋巴细胞的含量与肿瘤的恶性增生有一定关系，可做为恶性肿瘤免疫诊断的指标之一。
Objective: To explore the application of flow cytometry to detect the content of activated T lymphocytes in peripheral blood of patients with malignant tumors and the clinical value of this parameter in the diagnosis of malignant tumors. Methods: Flow cytometric immunoassay was used to quantitatively measure activated T cells in peripheral blood of 12 patients with malignant tumors. Results: The content of activated T cells in peripheral blood of malignant tumors was significantly higher than that of normal control group (P<0.05). Conclusion: The content of activated T lymphocytes has a certain relationship with the malignant proliferation of tumors, and it can be used as one of the indicators for the diagnosis of malignant tumors.