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以甲基丙烯酸缩水甘油酯为单体，乙二醇二甲基丙烯酸酯为交联剂在空管柱内就地 聚合制备了一种聚合物连续棒状色谱基质，并通过“在线”化学改性将其修饰为弱阳离子交换 柱。考察了该色谱柱的孔结构特征、表面亲水性能、对标准蛋白的分离效果和ｐＨ值对保留 行为的影响以及色谱柱的重现性。结果表明，该色谱柱对蛋白的分离性能及重现性良好，柱 寿命不短于半年。对溶菌酶的活性回收率达９６．２％。流动相流速为 ８．０ｍＬ／ｍｉｎ条件下，在 ３．５ ｍｉｎ内即可将５种蛋白完全分离
A polymer continuous rod chromatographic matrix was prepared by in situ polymerization of glycidyl methacrylate and ethylene glycol dimethacrylate as crosslinkers in an empty column. The polymer was modified by “in-line” chemical modification It is modified as a weak cation exchange column. The pore structure, surface hydrophilicity, the separation of standard proteins and the effect of pH value on the retention behavior and the reproducibility of the column were investigated. The results show that the separation performance and reproducibility of the column is good, and the column life is not less than six months. The activity of lysozyme recovery rate of 96.2%. With the mobile phase flow rate of 8.0mL / min, the five proteins could be completely separated within 3.5 minutes