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目的为提高医院感染败血症细菌培养阳性率及抗生素的有效使用.方法对我院1994年1月~1996年12月682例住院患者采取血液常规与高渗同步培养的结果进行对照分析.结果分离出细菌L型93株,而常规培养均为阴性,阳性率提高了13.6％.以金黄色葡萄球菌检出率最高,凝固酶阴性葡萄球菌次之,分别占检出菌的48.39％、37.63％.对青霉素、氨苄青霉素的耐药率较高,对红霉素、氯霉素耐药率在40％以下,少数对头孢类也产生了耐药性.结论采取同步培养可提高阳性率,为临床感染性疾病诊断和抗生素的合理使用提供了重要依据.“,”AIM To increase the positive rate of bacteria in specimers and the effective administeration of antibiotic in patients of nosoconial septicemia. METHODS The results of blood specimens in 682 from 1994 to 1996 with routine blood culture and hyperosmolality culture were analyzed in tandem. RESULTS 93 strains bacteria L form were separated from negative specimens from routine culture,the positive rate was increased by 13. 6%. The first was S. anareus, the second was coagulase - negative staphylococcus of 93 stains bacteria L form,which war 48. 39% and 37. 63% respectively. Drug sensitivity results showed that major atainswere resistant to Penicillin and Amipenicillin, low 40% Erythromycin and Chic - ramphenicol, a few to antibiotic. CONCLUSIONS Adoption step for step culture could not only increase positive rate,but also gave a important reason in the diagnosis of infection diseases and rational administeration of antibiotic.