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目的:探讨激光诱发小鼠脉络膜新生血管(CNV)后,骨髓间充质干细胞(BMSC)在其眼部的表型特征。方法:应用532nm倍频激光对C57BL/6J小鼠右眼进行光凝,建立CNV模型。将转染了绿色荧光蛋白(GFP)的骨髓间充质干细胞移植到视网膜下腔,应用荧光素眼底血管造影和组织病理学检查确定模型稳定后,对实验眼进行免疫荧光染色,观察GFP阳性细胞在眼部的表型特征。结果
Objective: To investigate the phenotypic characteristics of bone marrow mesenchymal stem cells (BMSCs) in the eye of mice induced by laser-induced choroidal neovascularization (CNV). Methods: The CNV model was established by photocoagulation of the right eye of C57BL / 6J mice with 532nm double frequency laser. Bone marrow-derived mesenchymal stem cells transfected with green fluorescent protein (GFP) were transplanted into the subretinal space. After the model was confirmed by fundus fluorescein angiography and histopathology, the experimental eyes were immunofluorescently stained to observe GFP positive cells Phenotypic characteristics in the eye. result