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目的研究呼吸道合胞病毒(RSV)感染豚鼠后肺组织β肾上腺素能受体(β受体)和Μ胆碱能受体(Μ受体)的变化。方法应用放射性配基结合分析法,测定病毒接种组和对照组豚鼠肺组织β、Μ受体密度和亲和力的变化。结果①RSV接种后豚鼠肺组织均出现毛细支气管炎、肺炎的病理改变。②β受体最大结合容量(Bmax)在RSV接种后无明显改变。β受体平衡解离常数(Kd)在RSV接种后3d增高,5~7d达高峰,以后逐渐下降,接种后14、21d与对照组比较差异无统计学意义。③高亲和力M受体亚型的Bmax和Kd值在感染前后无明显变化。低亲和力M受体亚型在RSV接种后3d出现Bmax增高,Kd值降低,5~7d达高峰,第21天与对照组差异有统计学意义。结论RSV感染可使β受体亲和力下降,低亲和力M受体密度增加、亲和力上升,两种受体系统之间的平衡失调是引起气道高反应性的机制之一。
Objective To study the changes of β adrenergic receptor (β receptor) and Μ cholinergic receptor (M receptor) in lung tissue of respiratory syncytial virus (RSV) infected guinea pigs. Methods Radioligand binding assay was used to determine the changes of β, M receptor density and affinity in the lung of guinea pigs inoculated with virus and control group. Results ① The pathological changes of bronchiolitis and pneumonia occurred in the lungs of guinea pigs after inoculation of RSV. ② The maximum binding capacity of β receptor (Bmax) did not change significantly after RSV inoculation. The equilibrium dissociation constant (Kd) of β receptor increased at 3 days after RSV inoculation and peaked at 5 to 7 days, then decreased gradually. There was no significant difference between the control group and 14 and 21 days after inoculation. ③ Bmax and Kd values of high affinity M receptor subtypes did not change significantly before and after infection. Low-affinity M receptor subtypes showed increased Bmax, Kd value on the 3rd day after RSV inoculation, and peaked on the 5th to 7th day. The difference was statistically significant on the 21st day and the control group. Conclusion RSV infection can reduce the affinity of β receptor, low affinity M receptor density increased, increased affinity, imbalance between the two receptor systems is one of the mechanisms leading to airway hyperresponsiveness.