论文部分内容阅读
目的 :探讨丹参酮ⅡA磺酸钠 (DS 2 0 1)对原代培养猪冠脉平滑肌上ATP敏感的的钾通道 (KATP)、钙激活钾通道 (KCa)的作用。方法 :采用膜片钳单通道电流记录技术进行定量研究。结果 :KATP(33 15pS)可被 30 μmol/L优降糖特异性阻断 ,而KCa(2 46 .5 3pS)则表现出明显的钙离子依赖性和TEA阻断作用等特点。内面向外式膜片下 ,浴液中加入DS 2 0 1可激活KATP及Ca。膜电位为 +5 0mV时 ,5、10、15 μmol/LDS 2 0 1分别使KATP开放概率 (NPo)从0 0 0 4± 0 0 0 1增加到 0 44 9± 0 0 0 4(n =5 ,P <0 0 1)、0 6 0 2± 0 0 32 (n =5 ,P <0 0 1)、0 90 4± 0 10 5 (n =5 ,P <0 0 1) ,2 0 μmol/LDS 2 0 1使KCa的NPo从 0 0 0 0增加到 0 0 11± 0 0 0 2 (n =3,P <0 0 1)。结论 :DS 2 0 1对KATP和KCa均有直接激活作用。
Objective: To investigate the effects of sodium tanshinone Ⅱ A sulfonate (DS 2 0 1) on ATP-sensitive potassium channel (KATP) and calcium-activated potassium channel (KCa) in porcine coronary arteries. Methods: Patch-clamp single-channel current recording technique was used for quantitative study. RESULTS: KATP (33 15 pS) was specifically blocked by 30 μmol / L of glyburide, whereas KCa (2 46.53 pS) showed obvious calcium-dependent and TEA-blocking effects. Inside the outward-facing diaphragm, adding DS 2 0 1 to the bath activates KATP and Ca. When the membrane potential was +5 0 mV, the open probability of KATP (NPo) increased from 0 0 0 4 ± 0 0 1 to 0 44 9 ± 0 0 0 1 respectively at 5,10,15 μmol / L DS 2 0 (n = 5, P <0.01), 0 6 0 2 ± 0 0 32 (n = 5, P 0 01), 0 90 4 ± 0 105 (n = 5, P 0 01) μmol / LDS 2 0 1 increased the NPo of KCa from 0 0 0 0 to 0 0 11 ± 0 0 0 2 (n = 3, P <0.01). Conclusion: DS 2 0 1 has direct activation on both KATP and KCa.