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目的观察旋毛虫肌幼虫在干燥和生理盐水环境中的生存状况。方法90只昆明小鼠被随机分为正常对照组、生理盐水组和干燥组,生理盐水组和干燥组分别再分为4组,共9组,每组10只。正常对照组每鼠经口感染200条收集的肌幼虫。生理盐水组分为4组:①肌幼虫0℃放置10 d,②肌幼虫20℃放置10 d,③肌幼虫0℃放置20 d,④肌幼虫20℃放置20 d;干燥组分组与生理盐水组相同。然后将8组小鼠每鼠经口感染200条处理的肌幼虫。感染后30 d处死小鼠,取膈肌压片镜检,并将全部肌肉人工消化后计数幼虫数。结果在生理盐水和干燥环境中放置10 d的肌幼虫分别感染小鼠后,压片法和人工消化法镜检,感染小鼠的幼虫检出率均为100%;在生理盐水和干燥环境中放置20 d的肌幼虫分别感染小鼠后,两种方法镜检,感染小鼠均未检出幼虫。结论在生理盐水和干燥环境中,肌幼虫能生存较长时间,但不超过20 d。
Objective To observe the survival of Trichinella spiralis larvae in dry and saline environment. Methods Totally 90 Kunming mice were randomly divided into normal control group, normal saline group and dry group. The normal saline group and dry group were divided into 4 groups, 9 in total, with 10 in each group. The normal control group was orally infected with 200 collected muscle larvae per mouse. The physiological saline solution was divided into 4 groups: ① larvae were placed at 0 ℃ for 10 days, ② larvae were placed at 20 ℃ for 10 days, ③ larvae were kept at 0 ℃ for 20 days, ④ larvae were stored at 20 ℃ for 20 days. The same group. Eight groups of mice were then orally infected with 200 treated muscle larvae. Mice were sacrificed 30 days after infection and microscopic examination of the diaphragm was performed. The number of larvae was counted after artificial digestion of all the muscles. Results After larvae were inoculated for 10 days in normal saline and dry environment respectively, the larvae infection rate in infected mice was 100% after being inoculated by the pelleting method and artificial digestion method. In normal saline and dry environment After 20 days of muscle larvae were infected with mice, the two methods microscopic examination, the infected mice were not detected larvae. Conclusion Muscle larvae can survive for longer periods of time in normal saline and dry environments but not longer than 20 days.