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Ascochlorin(Ⅲ)的产生菌Ascochyta viciae经亚硝基胍处理,得一变种N0.34,在27℃发酵7天,菌丝用氯仿-甲醇(9∶1)提取,提取液减压浓缩,残留物用正已烷提取,提取液冷冻去除Ⅲ的结晶。母液浓缩,经硅胶柱层析,以正已烷-丙酮(95∶5)洗脱得(Ⅰ),为无色针晶,分子式C_(23)H_(29)ClO_5,熔点84～85℃,[α]_D~(21)-50°(c=1,甲醇);再以正已烷-丙酮(4∶1)进一步洗脱得(Ⅱ),为无色针晶,分子式C_(23)H_(31)CIO_5,熔点75℃,[α]_D~(21)-7°(c=1,甲醇)。Ⅰ、Ⅱ和Ⅲ的紫外光谱[Ⅰ:228、290、350毫微米(20300、15000、820);Ⅱ:228、290、352毫微米
Ascochlorin (Ⅲ) producing strain Ascochyta viciae was treated with nitrosoguanidine to obtain a variety of N0.34, which was fermented at 27 ℃ for 7 days. The mycelium was extracted with chloroform-methanol (9:1), the extract was concentrated under reduced pressure and the residue Material was n-hexane extract, the extract was frozen to remove the crystal Ⅲ. The mother liquor was concentrated and purified by column chromatography on silica gel with n-hexane (95: 5) as eluent (I) as colorless needles. Molecular formula: C 23 H 29 ClO 5, m.p. 84-85 ° C, (II) as a colorless needle crystal, the compound of formula C_ (23) [C] H 31 CIO 5, melting point 75 ° C, [α] _D ~ (21) -7 ° (c = 1, methanol). UV spectra of Ⅰ, Ⅱ and Ⅲ [Ⅰ: 228,290,350 nm (20300,15000,820); II: 228,290,352 nm