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目的研究小窝蛋白-1(Caveolin-1)的表达与乙型肝炎病毒(HBV)感染以及相关肝细胞癌(HCC)的关系。方法免疫组化法检测HBV相关HCC中Caveolin-1表达量。构建Caveolin-1重组质粒,用Western blot检测转染了重组质粒的HepG2.2.15细胞中Caveolin-1表达量,并用ELISA法检测转染后培养上清中的HBsAg。结果免疫组化结果显示Caveolin-1在95%HBV相关HCC组织中表达低于癌旁组织(P<0.05)。Caveolin-1重组质粒构建成功并在HepG2.2.15中成功表达。ELISA检测发现用Caveolin-1重组质粒转染HBV感染稳定模型HepG2.2.15细胞48、72 h后,细胞培养上清中的HBsAg浓度相对未转染细胞分别下降了46.2%和48.8%(P<0.05)。重组质粒转染HBV感染急性细胞模型48、72 h后,HBsAg浓度相对未转染细胞分别下降了41%和50%(P<0.05)。结论 Caveolin-1在HBV相关HCC中呈低表达,Caveolin-1的高表达抑制了HBV HBsAg的分泌。
Objective To investigate the relationship between Caveolin-1 expression and hepatitis B virus (HBV) infection and related hepatocellular carcinoma (HCC). Methods The expression of Caveolin-1 in HBV-related HCCs was detected by immunohistochemistry. The recombinant plasmid Caveolin-1 was constructed. The expression of Caveolin-1 in HepG2.2.15 cells transfected with the recombinant plasmid was detected by Western blot. The HBsAg in the supernatant after transfection was detected by ELISA. Results The results of immunohistochemistry showed that the expression of Caveolin-1 in 95% HBV-related HCC tissues was lower than that in adjacent non-cancerous tissues (P <0.05). Caveolin-1 recombinant plasmid was successfully constructed and successfully expressed in HepG2.2.15. ELISA showed that HBsAg concentration in the cell culture supernatant decreased by 46.2% and 48.8% compared with untransfected cells (P <0.05, respectively) after transfection with HepG2.2.15 cells stably transfected with the recombinant plasmid of Caveolin-1 for 48 and 72 h ). The HBsAg concentration was reduced by 41% and 50% (P <0.05) respectively in untreated cells transfected with recombinant plasmids for 48 and 72 h. Conclusions Caveolin-1 is low expressed in HBV-related HCC and high expression of Caveolin-1 inhibits the secretion of HBV HBsAg.