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目的 采用体外细胞培养方法,研究不同浓度c9,t11 共轭亚油酸对人胃腺癌细胞(SGC- 790 1)的亚油酸代谢途径中限速酶———环氧合酶(COX -2 )及其产物前列腺素E2 (PGE2 )的影响。方法 采用逆转录聚合酶链反应(RT- PCR)和Westernblot方法检测不同浓度c9,t11 CLA处理后的SGC 790 1细胞中COX- 2mRNA和蛋白的表达,放射免疫方法检测细胞分泌的PGE2 浓度。结果 在2 5、5 0、10 0和2 0 0 μmol L浓度时,c9,t11 CLA均可下调COX 2mRNA和蛋白的表达,同时降低细胞中PGE2 的分泌。结论 环氧合酶2是c9,t11 CLA抑制肿瘤细胞增殖的作用靶点。
OBJECTIVE: To study the effects of different concentrations of c9 and t11 conjugated linoleic acid on the rate-limiting enzyme of cyclooxygenase (COX-2) in the metabolic pathway of human gastric adenocarcinoma cell line SGC-7901 ) And its product prostaglandin E2 (PGE2). Methods The expressions of COX-2 mRNA and protein in SGC-7901 cells treated with different concentrations of c9 and t11 CLA were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The concentrations of PGE2 secreted by cells were detected by radioimmunoassay. Results Both c9 and t11 CLA down-regulated the expression of COX-2 mRNA and protein and decreased the secretion of PGE2 in cells at 25, 50, 100 and 200 μmol L concentrations. Conclusions Cyclooxygenase 2 is the target of c9 and t11 CLA inhibiting tumor cell proliferation.