目前大多数研究者均使用显微镜下的机械法分离毛乳头,这种方法难度大,要求操作者具有熟练的技巧,这也是造成目前毛囊研究相对滞后的重要原因之一[1,2]。我科多年从事毛囊研究,对毛乳头的分离方法进行了不断探索和改进[3],从而形成“二步酶消化法”,该方法操作简便、毛乳头分离 At present, most researchers use the mechanical method to separate the dermal papilla under the microscope. This method is very difficult and requires the operator to have good skills. This is also one of the important causes of the current relative lag in hair follicle research [1,2]. Our department for many years engaged in hair follicles research, the method of separation of the dermal papillae has been continuously explored and improved [3], so as to form a “two-step enzyme digestion method”, the method is simple,