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目的观察康妇炎胶囊对盆腔炎性疾病后遗症模型大鼠组织形态学和免疫功能的影响,探讨其治疗盆腔炎性疾病后遗症的机制。方法取40只SD雌性大鼠用混合细菌加机械损伤法成功构建大鼠盆腔炎性疾病后遗症模型后,随机分为模型组和高、中、低3个剂量组,每组10只。另取20只正常SD雌性大鼠随机分为对照组和假手术组,每组10只,假手术组不损伤子宫内膜,只需往右侧子宫注射0.9%Na Cl 0.l mL。于造模后30 d,对照组、假手术组和模型组予以蒸馏水5 mL,灌胃给药;康妇炎胶囊高、中、低剂量组分别予以7.56×10~(-1),3.78×10~(-1),1.89×10-1g·kg~(-1)康妇炎胶囊,灌胃给药。6组大鼠均每天给药一次,连续20 d。比较6组大鼠的子宫指数和腹腔巨噬细胞吞噬功能。结果肉眼观察结果显示:模型组子宫明显肿胀、充血,积液充盈、黏连严重;中、高剂量组充血基本消失,少数有轻度积液,与盆腔周围组织黏连显著减轻。子宫组织病理学检查结果显示:模型组大鼠子宫壁组织结构紊乱,腺体明显减少,可见淋巴细胞、浆细胞浸润;中、高剂量组子宫壁结构基本接近正常,浆细胞减少、淋巴细胞,炎症不明显。对照组、假手术组、模型组和低、中、高3个剂量实验组的子宫指数分别为(0.39±0.16)×10~(-2),(0.28±0.15)×10~(-2),(0.16±0.06)×10~(-2),(0.22±0.05)×10~(-2),(0.27±0.20)×10~(-2),(0.31±0.12)×10~(-2)g·g~(-1);巨噬细胞吞噬百分率分别为(64.72±2.63)%,(64.04±3.07)%,(70.93±2.93)%,(71.16±2.11)%,(73.80±2.15)%,(74.48±2.72)%,中、高剂量组与模型组比较,差异均有统计学意义(均P<0.05)。结论康妇炎胶囊治疗盆腔炎性疾病作用机制可能与其参与免疫调节有关。
Objective To observe the effect of Kangfeiyan capsule on the histomorphology and immune function of the sequelae of pelvic inflammatory disease in rats and to explore its mechanism of sequelae of pelvic inflammatory disease. Methods Forty female SD rats were randomly divided into model group and high, middle and low dosage groups of 10 rats in each group with mixed bacterial and mechanical injury method to establish sequelae of pelvic inflammatory disease. Another 20 normal SD female rats were randomly divided into control group and sham operation group, with 10 rats in each group. The sham operation group did not injure the endometrium, only 0.9% Na Cl 0.l mL was injected into the right uterus. At 30 d after model establishment, 5 mL of distilled water was administrated in the control group, sham operation group and model group, respectively. The rats in Kangfeiyan capsule group were treated with 7.56 × 10 ~ (-1), 3.78 × 10 ~ (-1), 1.89 × 10-1g · kg ~ (-1) Kang Fuyan capsules, intragastric administration. Six groups of rats were administered once daily for 20 days. The uterus index and phagocytic function of peritoneal macrophages of 6 rats were compared. Results The macroscopic observation showed that the uterus in the model group was obviously swollen, hyperemia, fluid filling and severe adhesions. The hyperemia in the medium and high dose groups disappeared basically, and a few had mild effusion, which significantly reduced the adhesions with the pelvic tissues. Uterine histopathological examination showed that: the model group rats uterine wall structure disorder, glands significantly reduced, showing lymphocytes, plasma cells infiltration; medium and high dose group of uterine wall structure is almost normal, plasma cells decreased, lymphocytes, Inflammation is not obvious. The uterus index of the three experimental groups were (0.39 ± 0.16) × 10 ~ (-2) and (0.28 ± 0.15) × 10 ~ (-2) respectively in the control group, sham operation group, model group and low, , (0.16 ± 0.06) × 10 -2, (0.22 ± 0.05) × 10 -2, (0.27 ± 0.20) × 10 -2, (0.31 ± 0.12) × 10 ~ (- (64.72 ± 2.63)%, (64.04 ± 3.07)%, (70.93 ± 2.93)%, (71.16 ± 2.11)%, (73.80 ± 2.15 ) And (74.48 ± 2.72)%, respectively. There were significant differences between the model group and the medium and high dose groups (all P <0.05). Conclusion The mechanism of Kang Fuyan Capsule in treating pelvic inflammatory disease may be related to its involvement in immune regulation.