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本实验利用活体显微镜摄像技术、透射电镜技术，观察了内皮舒张因子（EDRF）对豚鼠耳蜗微循环的保护作用。结果提示：①速尿组（F）动物，经静脉注射药10min后，耳蜗微动脉缺血，血管内皮细胞损伤；②对速尿／L-精氨酸组（F／L－Arginine）动物，EDRF能使速尿引起的微动脉缺血明显改善，血管纹血管超微结构的损伤程度较单纯F组减轻；③速尿／L-硝基-精氨酸组（F／L－NNA）动物，耳蜗的缺血程度较F组加重。结论提示；EDRF能通过增加局部血流的灌注而改善和保护耳蜗微循环。本研究提供的实验结果，于临床开展微循环致聋疾病的治疗有所启迪。
In this study, we observed the protective effect of endothelium relaxing factor (EDRF) on cochlear microcirculation in guinea pigs by live microscopy and transmission electron microscopy. The results showed that: (1) In furosemide group (F) animals, ischemia of cochlear arteries and injury of vascular endothelial cells were observed after intravenous injection of 10 min; (2) F / L-Arginine group, EDRF can significantly reduce the rate of arteriolar ischemia induced by furosemide and reduce the ultrastructure damage of vascular pattern vessels compared with that of F group alone. (3) F / L-NNA animal , The degree of ischemia of the cochlea than the F group increased. Conclusions: EDRF can improve and protect cochlear microcirculation by increasing the perfusion of local blood flow. The experimental results provided by this study have some enlightenment for the treatment of deafness diseases in clinical practice.