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目的：建立肠系膜微血管的通透方程。方法：应用微循环活体观察和荧光示踪技术，通过计算机数字图像处理和自行研制的图像分析软件对荧光素钠（ＦｌＮａ）在肠系膜微血管的通透过程进行定量研究。结果：在注射荧光物质后早期的通透速度较快，当微血管周围组织中的荧光物质达到一定浓度后，通透速度明显减慢。通透过程呈两段式线性分布，每一时段内的通透速度恒定，第二时段的通透速度比第一时段慢约５倍。通透方程：ｔ＜２０ｓ：ｘ＝－３．４０６９＋６．０４５４×ｔ；ｔ＞２０ｓ：ｘ＝９９．４４５１＋１．０６２４×ｔ，通透速度：ｔ＜２０ｓ：Ｄ＝１．１６３５×１０－７ｃｍ２／ｓ；ｔ＞２０ｓ：Ｄ＝０．２０４５×１０－７ｃｍ２／ｓ。结论：本方法为进一步建立不同分子量的通透方程及通透速度与分子量的关系，以及定量研究体外培养的单层内皮细胞的通透性，从而达到全面、定量地测试及评价微循环物质交换参数的目的，提供了数学基础。
Objective: To establish the permeability equation of mesentery capillaries. Methods: Microvascular in vivo observation and fluorescence tracing technique were used to quantitatively study the permeation of sodium fluorescein (FlNa) in mesentery capillaries through computer digital image processing and self-developed image analysis software. Results: The early permeation rate was faster after the fluorescent substance was injected. When the fluorescent substance in the surrounding tissue of the microvascular reached a certain concentration, the permeation rate slowed down obviously. The process of permeation showed a two-stage linear distribution with a constant permeation rate in each time period. The permeation rate in the second period was about 5 times slower than that in the first period. The permeability equation: t <20s: x = -3.4069 + 6.0454 × t; t> 20s: x = 99.4451 + 1.0624 × t, the penetration velocity: t <20s: D = 1.1635 × 10-7cm2 /s;t>20s:D=0.2045×10-7cm2/s. Conclusion: This method is to further establish the permeability equation of different molecular weight and the relationship between the penetration rate and molecular weight, as well as quantitatively study the permeability of monolayer endothelial cells cultured in vitro, in order to achieve a comprehensive and quantitative test and evaluation of microcirculation exchange The purpose of the parameters provides the mathematical basis.