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Using Western blot and immunohistochemistry analysis, here the localization of RSG6 protein was determined in various tissues of rice. Western blot showed only a weak signal in mature pollen. Nevertheless, according to the result of immunohistochemistry with DAB and fluorescent staining, the expression of RSG6 protein appeared to begin at the bicellular microspore stage, and then keep activity in the sperm cells of mature pollens. The fluorescence pattern showed RSG6 polypeptide was present close to or attached to the surface of the isolated sperm cells. And this suggested that RSG6 might take an important part in the process of recognition of sperm cell and ovum.