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将合链霉亲和亲-蛋白A（Streptavidin－proteinA）融合蛋白基因的表达质粒pTSAPA导入大肠杆菌BL21（DE3），成功地表达了该融合蛋白，经SIJS－PAGE及免疫印迹等方法证实该蛋白既具有IgG的结合活性，又具生物素结合活性。
The recombinant plasmid pTSAPA was inserted into E.coli BL21 (DE3) to express the fusion protein. The fusion protein was confirmed by SIJS-PAGE and western blot. With IgG binding activity, but also with biotin-binding activity.