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枳幼苗SOD活性在细胞内的分布以细胞溶质部分最高,占总活性的86%左右,而对高温最敏感的则是叶绿体部分的SOD。用30~55℃一系列温度处理枳幼苗,随着处理温度的提高,先是叶绿体中的SOD的活性下降,膜脂过氧化产物MDA含量和细胞电解质泄漏率随之大幅度增加,枳幼苗出现热伤害症状。枳幼苗热伤害的相关原因是由于高温胁迫抑制了SOD的活性,削弱了对自由基清洗能力和对细胞膜的保护作用,从而加剧了膜脂过氧化作用,导致膜伤害,电解质大量泄漏,最终使枳幼苗表现热伤害症状。
The distribution of superoxide dismutase (SOD) activity in cytoplasm was highest in cytoplasm, accounting for about 86% of total activity, while the most sensitive to high temperature was chloroplast SOD. With a series of temperatures from 30 to 55 ℃, the growth of Trifoliate orange seedlings decreased with the increase of treatment temperature. First, the activities of SOD in chloroplasts decreased, the content of MDA and the leakage rate of cell electrolyte in the lipid peroxidation products increased significantly, Injury symptoms. The related causes of thermal injury in the seedlings of Hippophae rhamnoides are that high temperature stress inhibits the activity of SOD, weakens the ability to wash free radicals and protects the cell membrane, thereby exacerbating membrane lipid peroxidation, leading to membrane damage and large electrolyte leakage, Trifoliate seedlings show thermal injury symptoms.