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目的：进一步研究海马内微量注射（ｉｈ）褪黑素（ＭＴ）的免疫调节机制。方法：采用放射免疫法检测β－Ｅｎｄ和［３Ｈ］－ＴｄＲ参入法检测脾淋巴细胞增殖实验。结果：ｉｐ纳络酮（２．５ｍｇ·ｋｇ－１）能部分抑制ｉｈＭＴ的免疫调节作用，ｉｈＭＴ可明显提高下丘脑β－Ｅｎｄ水平，降低垂体的β－Ｅｎｄ。体外Ｎａｌ（０．２５ｎｍｏｌ·Ｌ－１～０．２５μｍｏｌ·Ｌ－１）对ｉｈＭＴ脾淋巴细胞增殖反应也呈剂量依赖性抑制作用，抗β－Ｅｎｄ抗体对ｉｈＭＴ增高的脾淋巴细胞增殖反应呈明显抑制作用。结论：ｉｈＭＴ的免疫调节作用可能主要与外周内源性阿片肽有关，而脾细胞产生的β－Ｅｎｄ可能在ｉｈＭＴ的免疫调节中发挥重要作用。
OBJECTIVE: To further investigate the immunomodulatory mechanism of melatonin (MT) in hippocampus by microinjection (ih). Methods: Radioimmunoassay was used to detect the proliferation of splenic lymphocytes by β-end and [3H] -TdR incorporation assay. Results: ip naloxone (2.5mg · kg-1) partially inhibited the immunomodulatory effect of ihMT. IhMT significantly increased β-end of hypothalamus and decreased β-end of pituitary. In vitro Nal (0.25nmol·L-1 ~ 0.25μmol·L-1) also inhibited the proliferation of ihMT splenic lymphocytes in a dose-dependent manner, and the anti-β-end antibody showed a significant proliferative response to splenic lymphocytes with increased ihMT Inhibition. CONCLUSION: The immunomodulatory effect of ihMT may be mainly related to the peripheral endogenous opioid peptide, while the β-end produced by spleen cells may play an important role in the immunomodulation of ihMT.