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目的探讨艾滋病病毒(HIV)和联合抗反转录病毒治疗(cART)对外周血单个核细胞(PBMC)中线粒体DNA含量的影响,及PBMC中线粒体DNA含量和脂肪代谢异常(LD)的关系。方法采用实时定量PCR检测未治疗、已经治疗的HIV感染者和正常对照组外周血单核细胞(PBMC)中线粒体DNA相对含量,比较不同人群PBMC中线粒体DNA有无差异,比较发生LD和未发生该症状患者线粒体DNA含量有无差异。结果未接受抗病毒治疗的HIV感染者和已治疗的HIV患者PBMC中线粒体DNA含量为非感染者的0.61倍和0.8倍,但无显著性差异(P=0.122)。发生LD患者PBMC中线粒体DNA相对含量(2-△△Ct)比无LD患者显著降低(0.57vs.1.12,P=0.045)。结论发生LD的患者血中PBMC中线粒体DNA含量显著下降,可能作为潜在临床辅助诊断指标,但需进一步研究。
Objective To investigate the effect of HIV and cART on mitochondrial DNA content in peripheral blood mononuclear cells (PBMCs) and the relationship between mitochondrial DNA content and fat metabolism abnormality (LD) in PBMCs. Methods The relative content of mitochondrial DNA in peripheral blood mononuclear cells (PBMCs) of untreated and treated HIV-infected persons and normal controls was detected by real-time PCR. The differences of mitochondrial DNA in peripheral blood mononuclear cells (PBMCs) from different groups were compared between LD and non-occurrence There is no difference in mitochondrial DNA content in this symptom. Results The mitochondrial DNA content of PBMCs in HIV-infected and untreated HIV-infected patients was 0.61-fold and 0.8-fold, respectively, non-infected with no significant difference (P = 0.122). The mitochondrial DNA content (2- △△ Ct) in PBMC of patients with LD was significantly lower than that of patients without LD (0.57 vs 1.12, P = 0.045). Conclusions The level of mitochondrial DNA in PBMC of patients with LD is significantly decreased, which may be used as a potential clinical diagnostic index, but further study is needed.