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为明确烟粉虱传播的番茄褪绿病毒(Tomato chlorosis virus,ToCV)与番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)对不同番茄品种的复合侵染情况,于2015年11月在山东省寿光市温室内采集13个番茄品种共390份疑似发病植株叶片,对不同番茄品种的TYLCV抗性和2种病毒的复合侵染以及温室内发病番茄植株上烟粉虱成虫的带毒率进行检测。结果表明,采集的13个番茄品种经分子标记检测鉴定均为TYLCV杂合抗性;不同番茄品种ToCV与TYLCV的复合侵染率存在明显差异,大果番茄粉宴和贝瑞上复合侵染率最高可达73.3%,而樱桃番茄八喜上未检测到这2种病毒的复合侵染。此外,在发病番茄植株上采集的烟粉虱成虫体内可检测到2种病毒,其中烟粉虱ToCV带毒率为90.7%,TYLCV带毒率为80.0%,同时检测到ToCV与TYLCV的概率为71.3%。表明ToCV和TYLCV的复合侵染在山东省番茄生产中普遍发生,烟粉虱可同时携带这2种病毒并广泛传播。
In order to clarify the complex infection of Tomato chlorosis virus (ToCV) and Tomato yellow leaf curl virus (TOLCV) to different tomato varieties transmitted by B. tabaci, 390 isolates from 13 tomato cultivars were collected from greenhouse in Shouguang City, Shandong Province. TYLCV resistance of two tomato cultivars and composite infection of two viruses and incidence of Bemisia tabaci adults on greenhouse tomato Test. The results showed that all the 13 tomato cultivars tested were identified as TYLCV by hybridization. The combined infection rates of ToCV and TYLCV were significantly different among different tomato cultivars. Up to 73.3%, while cherry tomato Baxi did not detect the composite infection of these two viruses. In addition, two kinds of viruses were detected in adults of B. tabaci collected from diseased tomato plants, of which the tolerance rate of ToCV to Becovirus was 90.7% and that of TYLCV was 80.0%, and the probability of ToCV and TYLCV was 71.3%. The results showed that the composite infection of ToCV and TYLCV was common in tomato production in Shandong Province. Bemisia tabaci transmitted the two viruses simultaneously and spread widely.